	US 12,461,004 B2
	Diffusion monitoring protocol for optimized tissue fixation
Daniel Bauer, Tucson, AZ (US); David Chafin, Tucson, AZ (US); and Michael Otter, Tucson, AZ (US)
Assigned to Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on Mar. 18, 2023, as Appl. No. 18/123,259.
Application 18/123,259 is a division of application No. 16/888,935, filed on Jun. 1, 2020, granted, now 11,639,886.
Application 16/888,935 is a division of application No. 15/624,700, filed on Jun. 15, 2017, granted, now 10,712,250, issued on Jul. 14, 2020.
Application 15/624,700 is a continuation of application No. PCT/EP2015/080254, filed on Dec. 17, 2015.
Claims priority of provisional application 62/097,520, filed on Dec. 29, 2014.
Claims priority of provisional application 62/093,151, filed on Dec. 17, 2014.
Claims priority of provisional application 62/093,173, filed on Dec. 17, 2014.
Prior Publication US 2023/0243726 A1, Aug. 3, 2023
Int. Cl. G01N 1/00 (2006.01); G01N 1/30 (2006.01); G01N 13/00 (2006.01); G01N 29/024 (2006.01); G01N 29/07 (2006.01); G01N 29/44 (2006.01)

CPC G01N 13/00 (2013.01) [G01N 1/30 (2013.01); G01N 29/024 (2013.01); G01N 29/07 (2013.01); G01N 29/44 (2013.01); G01N 2013/003 (2013.01); G01N 2291/011 (2013.01); G01N 2291/0245 (2013.01); G01N 2291/02475 (2013.01)]

17 Claims

1. A system for monitoring diffusion of a solution into a biological sample comprising:

(a) a signal analyzer containing one or more processors and one or more memories coupled to the one or more processors, wherein the one or more memories stores computer-executable instructions that cause the processor to perform operations comprising:

(a1) fitting a time of flight (TOF) trace to a single curve, the TOF trace comprising a plurality of TOF signal measurements transmitted through the biological sample at a plurality of time points, and

(a2) computing the rate of diffusion at each time point of the plurality of time points by calculating a derivative of the single curve.