| CPC F25B 25/005 (2013.01) [B01D 15/165 (2013.01); B01D 15/40 (2013.01); B04C 5/081 (2013.01); B04C 5/085 (2013.01); B04C 9/00 (2013.01); C07C 45/79 (2013.01); C07C 51/47 (2013.01); C07C 67/56 (2013.01); C07C 231/24 (2013.01); C09K 5/041 (2013.01); F25B 7/00 (2013.01); F25B 9/002 (2013.01); F25B 9/008 (2013.01); F25B 9/10 (2013.01); F25B 49/02 (2013.01); G01N 30/30 (2013.01); G01N 30/54 (2013.01); B04C 2009/004 (2013.01); C09K 2205/106 (2013.01); C09K 2205/13 (2013.01); C09K 2205/132 (2013.01); F25B 2600/2513 (2013.01); F25B 2700/21171 (2013.01); G01N 30/20 (2013.01); Y02P 20/54 (2015.11)] | 20 Claims |

|
20. A pressurized fluid chromatography method using a pressure-equalizing vessel, said method comprising:
inserting a cylindrical inner column into a cylindrical outer column of a pressure-equalizing vessel of a pressurized fluid chromatography system, the cylindrical inner column being an insertable and removable chromatography cartridge containing stationary phase media;
creating, a cylindrical interspace region between an inner diameter of the cylindrical outer column and the outer diameter of the cylindrical inner column upon insertion of the cylindrical inner column;
concurrently filling, with a fluid pressurized to at least 500 psi (35 bar), an internal space of the cylindrical inner column and the interspace region;
maintaining a pressure differential of less than about 200 psi between the internal space of the cylindrical inner column and the interspace region;
preventing fluid communication between the interspace region and the cylindrical internal space of the cylindrical inner column; and
wherein the pressure-equalizing vessel is filled with said fluid from an upstream source that is split into two streams, one stream flowing into the interspace region and the other stream flowing into the internal space of the inner column of the pressure-equalizing vessel.
|