US 12,460,319 B2
Tissue spatial omics
Jocelyn Kishi, Watertown, MA (US)
Assigned to Digital Biology Inc., Watertown, MA (US)
Filed by Digital Biology Inc., Watertown, MA (US)
Filed on Apr. 23, 2024, as Appl. No. 18/643,523.
Application 18/643,523 is a continuation of application No. PCT/US2023/064873, filed on Mar. 23, 2023.
Claims priority of provisional application 63/346,454, filed on May 27, 2022.
Claims priority of provisional application 63/326,384, filed on Apr. 1, 2022.
Claims priority of provisional application 63/323,290, filed on Mar. 24, 2022.
Prior Publication US 2024/0263169 A1, Aug. 8, 2024
Int. Cl. C40B 50/06 (2006.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6881 (2018.01)
CPC C40B 50/06 (2013.01) [C12N 15/1065 (2013.01); C12N 15/1096 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6881 (2013.01)] 29 Claims
OG exemplary drawing
 
1. A method of biological cell spatial information generation, the method comprising:
depositing DNA barcodes onto a population of cells, wherein the DNA barcodes hybridize to a target region, and wherein the DNA barcodes comprise a photo-reactive nucleobase capable of crosslinking to another nucleobase, wherein the population of cells is present on a synthetic substrate;
selectively radiating the population of cells at a region of interest (ROI) to form covalently linked DNA barcode-target complexes, wherein the radiating is selectively varied by intensity or duration;
washing the population of cells to remove the DNA barcodes that are not complexed to the target region; and
repeating the depositing, selectively radiating, and washing steps, wherein the DNA barcodes in a first of the depositing steps comprise a different sequence than the DNA barcodes in a second of the depositing steps.