US 12,460,318 B2
Methods for determining lymphocyte receptor chain pairs
Carl Lars Genghis Hansen, Vancouver (CA); Georgia Elizabeth Mewis, Vancouver (CA); Kevin Albert Heyries, Vancouver (CA); Michael Andrew Vaninsberghe, Vancouver (CA); Daniel Jay Da Costa, Pitt Meadows (CA); and Marketa Ricicova, Vancouver (CA)
Assigned to THE UNIVERSITY OF BRITISH COLUMBIA, Vancouver (CA)
Filed by The University of British Columbia, Vancouver (CA)
Filed on Jun. 8, 2022, as Appl. No. 17/835,220.
Application 17/835,220 is a division of application No. 16/423,861, filed on May 28, 2019, granted, now 11,371,166.
Application 16/423,861 is a division of application No. 15/312,909, granted, now 10,400,281, issued on Sep. 3, 2019, previously published as PCT/CA2015/000328, filed on May 22, 2015.
Claims priority of provisional application 62/002,152, filed on May 22, 2014.
Prior Publication US 2023/0041481 A1, Feb. 9, 2023
Int. Cl. C40B 30/04 (2006.01); C07K 16/00 (2006.01)
CPC C40B 30/04 (2013.01) [C07K 16/00 (2013.01)] 19 Claims
OG exemplary drawing
 
1. A method for identifying a plurality of T-cell receptor chain pairs in a sample comprising a plurality of T-cells or progeny thereof, wherein each of the T-cell receptor chain pairs is from a single T-cell clone, comprising:
partitioning the sample into a plurality of individual vessels to provide a plurality of sample subpopulations;
sequencing nucleic acid clonotypes encoding the T-cell receptor chains in each subpopulation of the plurality of sample subpopulations to determine the identity of the T-cell receptor chains in each sample subpopulation;
determining the observed distribution of each of the T-cell receptor chains across the sample subpopulations and calculating statistical probabilities that the observed distributions of T-cell receptor chain pairs in the sample subpopulations are independent from one another;
identifying the plurality of T-cell receptor chain pairs based on the calculated statistical probabilities.