US 12,460,272 B2
Compositions and methods for amplifying or detecting varicella-zoster virus
Marcela Alejandra Carvallo Pinto, San Diego, CA (US); Amber Jean Hillius, San Diego, CA (US); and Ankur Shah, San Diego, CA (US)
Assigned to GEN-PROBE INCORPORATED, San Diego, CA (US)
Appl. No. 17/281,543
Filed by GEN-PROBE INCORPORATED, San Diego, CA (US)
PCT Filed Oct. 1, 2019, PCT No. PCT/US2019/053943
§ 371(c)(1), (2) Date Mar. 30, 2021,
PCT Pub. No. WO2020/072409, PCT Pub. Date Apr. 9, 2020.
Claims priority of provisional application 62/739,571, filed on Oct. 1, 2018.
Prior Publication US 2022/0017980 A1, Jan. 20, 2022
Int. Cl. C12Q 1/70 (2006.01)
CPC C12Q 1/705 (2013.01) 15 Claims
 
1. A composition for amplifying a Varicella-Zoster Virus (VZV) target nucleic acid sequence and detecting an amplified VZV target nucleic acid sequence comprising:
(a) a forward amplification primer 19-50 nucleobases in length and comprising the nucleobase sequence of SEQ ID NO: 1, 2, 3, 4, 5, 6, or 7; and
(b) a reverse amplification primer 19-50 nucleobases in length and comprising the nucleobase sequence of SEQ ID NO: 16, 17, 18, 19, 20, 21, or 22, wherein:
(i) (a) comprises SEQ ID NO: 1 and (b) comprises SEQ ID NO: 16 or 17;
(ii) (a) comprises SEQ ID NO: 2 and (b) comprises SEQ ID NO: 17;
(iii) (a) comprises SEQ ID NO: 3 and (b) comprises SEQ ID NO: 18;
(iv) (a) comprises SEQ ID NO: 4 and (b) comprises SEQ ID NO: 19;
(v) (a) comprises SEQ ID NO: 5 and (b) comprises SEQ ID NO: 20;
(vi) (a) comprises SEQ ID NO: 6 and (b) comprises SEQ ID NO: 21; or
(vii) (a) comprises SEQ ID NO: 7 and (b) comprises SEQ ID NO: 22; and
(c) a detection probe comprising a detectable label.