US 12,460,271 B2
Method for detecting specific DNA in a sample
Tomoji Mashimo, Suita (JP); Kazuto Yoshimi, Suita (JP); and Satomi Shibumura, Suita (JP)
Assigned to C4U CORPORATION, Suita (JP); and OSAKA UNIVERSITY, Suita (JP)
Appl. No. 17/794,704
Filed by C4U CORPORATION, Suita (JP); and OSAKA UNIVERSITY, Suita (JP)
PCT Filed Jan. 25, 2021, PCT No. PCT/JP2021/002372
§ 371(c)(1), (2) Date Jul. 22, 2022,
PCT Pub. No. WO2021/149829, PCT Pub. Date Jul. 29, 2021.
Claims priority of application No. 2020-010216 (JP), filed on Jan. 24, 2020; and application No. 2020-086698 (JP), filed on May 18, 2020.
Prior Publication US 2023/0099483 A1, Mar. 30, 2023
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/70 (2006.01); C12Q 1/683 (2018.01); C12Q 1/6844 (2018.01)
CPC C12Q 1/701 (2013.01) [C12Q 1/68 (2013.01); C12Q 1/683 (2013.01); C12Q 1/6844 (2013.01); C12Q 2600/156 (2013.01)] 1 Claim
OG exemplary drawing
 
1. A method for detecting a specific DNA in a sample comprising the steps of:
(a) contacting the sample with a CRISPR-Cas3 system targeting the specific DNA and a single-stranded probe DNA; and
(b) detecting cleavage of the single-stranded probe DNA by the CRISPR-Cas3 system that occurs in a case where the specific DNA is present in the sample.