US 12,460,258 B2
Nucleic acid testing device and nucleic acid testing method
Ryo Yonehara, Saitama (JP); and Ayako Michida, Gunma (JP)
Assigned to PHC Holdings Corporation, Tokyo (JP)
Filed by PHC Holdings Corporation, Tokyo (JP)
Filed on Oct. 7, 2022, as Appl. No. 17/961,975.
Application 17/961,975 is a continuation of application No. PCT/JP2021/009033, filed on Mar. 8, 2021.
Prior Publication US 2023/0023097 A1, Jan. 26, 2023
Int. Cl. C12Q 1/6858 (2018.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01)
CPC C12Q 1/6858 (2013.01) [B01L 3/502715 (2013.01); B01L 7/52 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/18 (2013.01); C12Q 2600/156 (2013.01)] 7 Claims
 
1. A nucleic acid testing device, comprising:
a stage on which is placed a tissue section to which a solution has been added, the solution containing a labeling substance of a target nucleic acid and an amplification reagent for the target nucleic acid;
a temperature adjuster that adjusts the temperature of the tissue section on the stage;
a temperature controller that controls the temperature adjuster to advance nucleic acid amplification reaction in the tissue section;
an intensity detector that detects label intensity in the tissue section over time;
a storage unit that stores detection information generated by the intensity detector;
at least one of a region setting unit that determines a test region in the tissue section on the detection information based on at least one of a shape of the tissue section or label intensity in the tissue section, or an accepting unit that accepts specification, from a user, of a test region in the tissue section on the detection information; and
an information processing unit that derives a change over time of the label intensity in the test region and generates information regarding the target nucleic acid in the test region based on the change over time of the label intensity in the test region,
wherein the information processing unit is configured to calculate a ratio between a positive region, in which label intensity is greater than or equal to a predetermined threshold, and a negative region, in which label intensity is less than the threshold, in the test region.