US 12,460,248 B2
Method and system for sampling material from cells
Arkadiusz Bibillo, Walnut Creek, CA (US); Pranav Patel, Fremont, CA (US); Christopher Reggiardo, Castro Valley, CA (US); and Jonathan Petersen, Sunnyvale, CA (US)
Assigned to Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed by Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed on Jan. 2, 2023, as Appl. No. 18/149,143.
Application 18/149,143 is a continuation of application No. 17/105,333, filed on Nov. 25, 2020, granted, now 11,542,541.
Claims priority of provisional application 62/940,866, filed on Nov. 26, 2019.
Prior Publication US 2023/0366005 A1, Nov. 16, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6853 (2018.01); C12Q 1/6874 (2018.01)
CPC C12Q 1/6806 (2013.01) [C12N 15/1003 (2013.01); C12Q 1/6853 (2013.01); C12Q 1/6874 (2013.01)] 8 Claims
OG exemplary drawing
 
1. A method of sampling nucleic acid material from tissue, the method comprising:
receiving a tissue section on a surface of a chip, wherein the chip includes an electrode array of sampling electrodes arranged along the surface, wherein a well array of wells is formed in the surface of the chip, wherein each sampling electrode of the electrode array is located at the bottom of a different well of the well array, and wherein each tag of the tag array is located in one of the wells of the well array;
measuring an electrical property for each sampling electrode of the electrode array;
applying an individually controllable lysis voltage to the sampling electrode if the measured electrical property for the sampling electrode meets one or more predefined criteria; and
applying an electrophoresis voltage to the sampling electrode, if the individually controllable lysis voltage was applied to the sampling electrode, to drive nucleic acids, if any, that were released from one of the cells of the tissue section by applying the lysis voltage, to copies of a tag of the tag array that is aligned with the sampling electrode.