US 12,460,245 B2
Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
Lance P. Encell, Fitchburg, WI (US); Mary P. Hall, Waunakee, WI (US); Keith V. Wood, Madison, WI (US); and Monika G. Wood, Madison, WI (US)
Assigned to Promega Corporation, Madison, WI (US)
Filed by Promega Corporation, Madison, WI (US)
Filed on Mar. 1, 2022, as Appl. No. 17/683,939.
Application 17/683,939 is a continuation of application No. 16/725,586, filed on Dec. 23, 2019, granted, now 11,293,047.
Application 16/725,586 is a continuation of application No. 15/644,876, filed on Jul. 10, 2017, granted, now 10,550,420, issued on Feb. 4, 2020.
Application 15/644,876 is a continuation of application No. 14/851,608, filed on Sep. 11, 2015, granted, now 9,732,373, issued on Aug. 15, 2017.
Claims priority of provisional application 62/049,150, filed on Sep. 11, 2014.
Prior Publication US 2022/0186287 A1, Jun. 16, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/66 (2006.01); C12N 9/02 (2006.01); G01N 21/64 (2006.01); G01N 33/542 (2006.01); G01N 33/68 (2006.01)
CPC C12Q 1/66 (2013.01) [C12N 9/0069 (2013.01); G01N 21/6428 (2013.01); G01N 33/542 (2013.01); G01N 33/6803 (2013.01); C12Y 113/12007 (2013.01); G01N 2021/6432 (2013.01); G01N 2021/6439 (2013.01); G01N 2333/90241 (2013.01)] 25 Claims
 
1. An isolated polynucleotide encoding a click beetle red luciferase (CBR) variant polypeptide having at least 90% amino acid sequence identity to SEQ ID NO: 1 and consisting of an amino acid substitution at a position corresponding to position 348 of SEQ ID NO: 1, wherein the variant CBR polypeptide has at least one of enhanced luminescence, altered light emission wavelength, altered substrate specificity, or a combination thereof, as compared to a CBR polypeptide of SEQ ID NO: 1, wherein the amino acid substitution comprises Q348H or Q348E.