US 12,460,241 B2
Biosensor
Dong Hee Ma, Gyeonggi-do (KR); and Dong Yeop Lee, Gyeonggi-do (KR)
Assigned to DONGWOO FINE-CHEM CO., LTD., Jeollabuk-do (KR)
Filed by DONGWOO FINE-CHEM CO., LTD., Jeollabuk-do (KR)
Filed on Apr. 12, 2022, as Appl. No. 17/718,626.
Application 17/718,626 is a continuation of application No. PCT/KR2020/016362, filed on Nov. 19, 2020.
Prior Publication US 2022/0243245 A1, Aug. 4, 2022
Int. Cl. C12Q 1/00 (2006.01); C12Q 1/32 (2006.01); G01N 27/327 (2006.01)
CPC C12Q 1/005 (2013.01) [C12Q 1/32 (2013.01); G01N 27/3272 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A biosensor, comprising:
a substrate;
a working electrode disposed on the substrate, the working electrode comprising:
a sensing electrode disposed on a top surface of the substrate;
an enzyme reaction layer disposed on a top surface of the sensing electrode; and
a permeation control layer disposed on a top surface of the enzyme reaction layer, the permeation control layer comprising a water-soluble polymer and a water-insoluble polymer; and
a reference electrode disposed on the substrate to be spaced apart from the working electrode, so that the permeation control layer is spaced apart from the reference electrode,
wherein the permeation control layer has a multi-layered structure from a double-layered structure to a penta-layered structure,
wherein the permeation control layer is formed from a composition comprising a solvent including water, and a mixture of the water-soluble polymer and the water-insoluble polymer,
the water-soluble polymer is included in an amount from 1 wt % to 10 wt % based on a total weight of the composition, and
the water-insoluble polymer is included in an amount from 0.5 wt % to 5 wt % based on a total weight of the composition.