US 12,460,238 B2
Methods of producing compositions comprising hydrophilic sophorolipids
Sean Farmer, Ft. Lauderdale, FL (US); Ken Alibek, Solon, OH (US); Amir Mahmoudkhani, Solon, OH (US); and Janaina Izabel Da Silva De Aguiar, Solon, OH (US)
Assigned to Locus Solutions IPCo, LLC, Solon, OH (US)
Appl. No. 17/769,951
Filed by Locus Solutions IPCo, LLC, Solon, OH (US)
PCT Filed May 20, 2021, PCT No. PCT/US2021/033352
§ 371(c)(1), (2) Date Apr. 18, 2022,
PCT Pub. No. WO2021/236904, PCT Pub. Date Nov. 25, 2021.
Claims priority of provisional application 63/027,676, filed on May 20, 2020.
Prior Publication US 2023/0304060 A1, Sep. 28, 2023
Int. Cl. C12P 19/44 (2006.01)
CPC C12P 19/44 (2013.01) 8 Claims
OG exemplary drawing
 
1. A method for producing a sophorolipid composition with an HLB of 9 or greater, the method comprising:
obtaining a yeast fermentation product comprising a mixture of hydrophilic SLP molecules comprising non-acetylated linear SLP, and hydrophobic SLP molecules comprising lactonic SLP and mono-and di-acetylated linear SLP, said mixture being produced by cultivating a SLP-producing microorganism;
functionalizing the hydrophobic SLP molecules by converting them into hydrophilic molecules to produce a hydrophilic SLP fraction comprising only hydrophilic SLP molecules, wherein the hydrophobic SLP molecules are converted to hydrophilic SLP molecules by mixing the hydrophobic SLP molecules with a base;
producing a purified hydrophobic SLP fraction; and
mixing the hydrophilic SLP fraction with the purified hydrophobic SLP fraction at a ratio of hydrophilic SLP molecules to purified hydrophobic SLP molecules between 60:40 to 75:25.