US 12,460,237 B2
Trehalose-rich yeast extract
Dominik Spickermann, Hamburg (DE); Mariët van der Werf, Hamburg (DE); Cornelia Reimers, Hamburg (DE); and Catharina Kleist, Hamburg (DE)
Assigned to OHLY GmbH, Hamburg (DE)
Appl. No. 17/614,707
Filed by OHLY GmbH, Hamburg (DE)
PCT Filed May 29, 2020, PCT No. PCT/EP2020/065065
§ 371(c)(1), (2) Date Nov. 29, 2021,
PCT Pub. No. WO2020/240012, PCT Pub. Date Dec. 3, 2020.
Claims priority of application No. 19177208 (EP), filed on May 29, 2019.
Prior Publication US 2022/0228181 A1, Jul. 21, 2022
Int. Cl. C12P 19/12 (2006.01); A23L 2/60 (2006.01); A23L 27/30 (2016.01); A23L 33/125 (2016.01); A23L 33/145 (2016.01); A61K 8/60 (2006.01); A61K 8/9728 (2017.01); A61Q 19/00 (2006.01)
CPC C12P 19/12 (2013.01) [A23L 2/60 (2013.01); A23L 27/33 (2016.08); A23L 33/125 (2016.08); A23L 33/145 (2016.08); A61K 8/60 (2013.01); A61K 8/9728 (2017.08); A61Q 19/00 (2013.01); A61K 2800/85 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for preparing a trehalose-rich yeast extract comprising a trehalose content of at least 15% (w/w) based on dry matter, said method comprising
(a) fermenting yeast cells in a suitable medium;
(b) subjecting the yeast cells to abiotic stress;
(c) subjecting the yeast cells to a temperature of at least 70° C. to inactivate yeast enzymes;
(d) incubating the yeast cells with a least one peptidase enzyme;
(e) removing non-soluble cell components after peptidase treatment; and
(f) obtaining the trehalose-rich yeast extract,
wherein the method does not include a trehalose extraction step that makes use of any organic solvents or acids.