	US 12,460,236 B2
	Fructose-4-epimerase and method for preparing tagatose using same
Young Mi Lee, Seoul (KR); Eul-Soo Park, Seoul (KR); Sun Mi Shin, Seoul (KR); Seong Bo Kim, Seoul (KR); and Eun Jung Choi, Seoul (KR)
Assigned to CJ CHEILJEDANG CORPORATION, Seoul (KR)
Appl. No. 17/635,157
Filed by CJ CHEILJEDANG CORPORATION, Seoul (KR)
PCT Filed Aug. 13, 2020, PCT No. PCT/KR2020/010725 § 371(c)(1), (2) Date Aug. 9, 2022, PCT Pub. No. WO2021/029688, PCT Pub. Date Feb. 18, 2021.
Claims priority of application No. 10-2019-0099815 (KR), filed on Aug. 14, 2019.
Prior Publication US 2022/0380818 A1, Dec. 1, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 19/02 (2006.01); C12N 9/90 (2006.01); C12P 19/24 (2006.01)

CPC C12P 19/02 (2013.01) [C12N 9/90 (2013.01); C12P 19/24 (2013.01); C12Y 501/03 (2013.01)]

12 Claims

1. A fructose-4-epimerase variant, comprising substitutions of amino acid residues corresponding to positions 97, 124, 367, and 390 from a N-terminus of a fructose-4-epimerase comprising the amino acid sequence of SEQ ID NO: 1 with other amino acids.