	US 12,460,227 B2
	Single plasmid vector system for packaging recombinant human adenovirus type 4 and application thereof
Wei Chen, Beijing (CN); Busen Wang, Beijing (CN); Lihua Hou, Beijing (CN); Shipo Wu, Beijing (CN); Qiang Guo, Beijing (CN); Zhe Zhang, Beijing (CN); Zhenghao Zhao, Beijing (CN); Jinlong Zhang, Beijing (CN); Ling Fu, Beijing (CN); and Xiaohong Song, Beijing (CN)
Assigned to Academy of Military Medical Science, PLA, Beijing (CN)
Appl. No. 17/629,592
Filed by ACADEMY OF MILITARY MEDICAL SCIENCE, PLA, Beijing (CN)
PCT Filed Sep. 26, 2019, PCT No. PCT/CN2019/108318 § 371(c)(1), (2) Date Jan. 24, 2022, PCT Pub. No. WO2021/017138, PCT Pub. Date Feb. 4, 2021.
Claims priority of application No. 201910685065.7 (CN), filed on Jul. 27, 2019.
Prior Publication US 2022/0315949 A1, Oct. 6, 2022
Int. Cl. C12N 15/86 (2006.01); C12N 7/00 (2006.01)

CPC C12N 15/86 (2013.01) [C12N 7/00 (2013.01); C12N 2710/10321 (2013.01); C12N 2710/10343 (2013.01); C12N 2710/10352 (2013.01); C12N 2710/10364 (2013.01)]

8 Claims

1. A single-plasmid vector system for packaging recombinant human adenovirus type 4, wherein the vector system contains

an E3-deleted human adenovirus type 4 genome comprising a packaging signal and an E1 region,

a pBR322 replication origin,

a kanamycin resistance gene, and

a nucleotide sequence consisting of SEQ ID NO: 1 which comprises a site for insertion of an exogenous gene, and said SEQ ID NO:1 is located after the packaging signal and prior to the E1 region,

wherein a PmeI restriction enzyme site is located at 447-454 bp of SEQ ID NO: 1 for insertion of an exogenous gene.