US 12,460,224 B2
Modified promoter of a parthenogenesis gene
Rik Hubertus Martinus Op Den Camp, Wageningen (NL); and Peter Johannes Van Dijk, Wageningen (NL)
Assigned to Keygene N.V., Wageningen (NL)
Filed by Keygene N.V., Wageningen (NL)
Filed on Apr. 11, 2023, as Appl. No. 18/133,392.
Application 18/133,392 is a continuation of application No. PCT/EP2021/078281, filed on Oct. 13, 2021.
Claims priority of application No. 20201578 (EP), filed on Oct. 13, 2020.
Prior Publication US 2023/0383308 A1, Nov. 30, 2023
Int. Cl. C12N 15/82 (2006.01); A01H 1/02 (2006.01)
CPC C12N 15/8287 (2013.01) [A01H 1/022 (2021.01)] 20 Claims
 
1. A method of producing a mutant gene capable of inducing a parthenogenesis phenotype, the method comprising:
(a) providing a gene comprising a sequence encoding a PAR protein of SEQ ID NO: 1, 6 or 11, or any orthologue thereof, operably linked to a promoter comprising one or more transcription factor MYB binding sites, wherein the PAR protein is characterized in that it comprises a zinc finger C2H2-type domain having the consensus amino acid sequence of SEQ ID NO: 37 and an EAR motif having the consensus amino acid sequence of SEQ ID NO: 58 or 59; and
(b) modifying the promoter by modifying the sequence upstream of the one or more MYB binding sites to increase transcription of the encoded PAR protein, wherein the modifying comprises at least one of:
inserting a sequence of between 50 and 2000 base pairs; and
deleting a sequence of between 10 and 1000 base pairs,
and wherein said insertion and/or deletion is at most 200 base pairs upstream of the MYB binding site of the one or more MYB binding site located most upstream from the coding sequence.