	US 12,460,221 B2
	Method for the production of high levels of PUFA in plants
Toralf Senger, Research Triangle Park, NC (US); Hui Yang, Saskatoon (CA); Carl Andre, Research Triangle Park, NC (US); and Patricia Vrinten, Saskatoon (CA)
Assigned to BASF PLANT SCIENCE COMPANY GMBH, Ludwigshafen (DE)
Appl. No. 17/273,858
Filed by BASF PLANT SCIENCE COMPANY GMBH, Ludwigshafen (DE)
PCT Filed Sep. 6, 2019, PCT No. PCT/EP2019/073831 § 371(c)(1), (2) Date Mar. 5, 2021, PCT Pub. No. WO2020/049155, PCT Pub. Date Mar. 12, 2020.
Claims priority of application No. 18193296 (EP), filed on Sep. 7, 2018; and application No. 18193841 (EP), filed on Nov. 9, 2018.
Prior Publication US 2023/0183728 A1, Jun. 15, 2023
Int. Cl. C12N 15/82 (2006.01); A23D 9/00 (2006.01); A23L 33/115 (2016.01); C07K 16/40 (2006.01); C12N 9/12 (2006.01)

CPC C12N 15/8247 (2013.01) [A23D 9/00 (2013.01); A23L 33/115 (2016.08); C07K 16/40 (2013.01); C12N 9/1288 (2013.01); C12N 15/8218 (2013.01); C12Y 207/08002 (2013.01); A23V 2002/00 (2013.01)]

5 Claims

1. A method for increasing the delta-6 elongase conversion efficiency in an oil crop plant of a Brassica species that produces very long chain polyunsaturated fatty acids (vlcPUFAs) and expresses a delta-6 elongase, comprising:

providing an oil crop plant of a Brassica species that expresses a delta-6 elongase and a delta-6 desaturase and that has decreased activity, compared to a control plant, of one or more phosphotidylcholine:diacylglycerol cholinephosphotransferase (PDCT) selected from the group consisting of:

(a) PDCT3 and/or PDCT5 having at least 80% sequence identity with SEQ ID NO: 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60;

(b) PDCT3 and/or PDCT5 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59; and

(c) PDCT3 and/or PDCT5 encoded by a polynucleotide that differs from SEQ ID NO: 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59 due to the degeneracy of the genetic code

wherein the plant produces docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and docosapentaenoic acid (DPA).