US 12,460,218 B2
Parthenogenetic haploid induction gene DMP and application thereof
Shaojiang Chen, Beijing (CN); Yu Zhong, Beijing (CN); Chenxu Liu, Beijing (CN); Xiaolong Qi, Beijing (CN); Mengran Li, Beijing (CN); Baojian Chen, Beijing (CN); Yanyan Jiao, Beijing (CN); and Zongkai Liu, Beijing (CN)
Assigned to CHINA AGRICULTURAL UNIVERSITY, Beijing (CN)
Appl. No. 17/595,768
Filed by CHINA AGRICULTURAL UNIVERSITY, Beijing (CN)
PCT Filed Jul. 1, 2020, PCT No. PCT/CN2020/099680
§ 371(c)(1), (2) Date Nov. 23, 2021,
PCT Pub. No. WO2020/239137, PCT Pub. Date Dec. 3, 2020.
Claims priority of application No. 201910445082.3 (CN), filed on May 27, 2019.
Prior Publication US 2022/0403400 A1, Dec. 22, 2022
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8213 (2013.01) [C12N 15/8218 (2013.01); C12N 15/8287 (2013.01); C12N 2310/20 (2017.05)] 8 Claims
 
1. A method for preparing a plant haploid inducer line is as follows A1) or A2):
A1) knocking out the gene AtDMP8 and gene AtDMP9 in the Arabidopsis thaliana genome to obtain a transgenic Arabidopsis thaliana, namely the Arabidopsis thaliana haploid inducer line;
A2) knocking out the gene SIDMP in the tomato genome to obtain a transgenic tomato, namely the tomato haploid inducer line; wherein
the protein encoded by the gene AtDMP8 is a protein shown in SEQ ID NO: 2, or a protein having 85% or more identity with the amino acid sequence shown in SEQ ID NO. 2and having the same function, or a fusion protein obtained by attaching a tag to the N-terminus and/or C-terminus of the protein shown in SEQ ID NO. 2;
the protein encoded by the gene AtDMP9 is a protein shown in SEQ ID NO: 4, or a protein having 85% or more identity with the amino acid sequence shown in SEQ ID NO. 4 and having the same function, or a fusion protein obtained by attaching a tag to the N-terminus and/or C-terminus of the protein shown in SEQ ID NO. 4; and
the protein encoded by the gene SIDMP is a protein shown in SEQ ID NO: 6, or a protein having 85% or more identity with the amino acid sequence shown in SEQ ID NO. 6 and having the same function, or a fusion protein obtained by attaching a tag to the N-terminus and/or C-terminus of the protein shown in SEQ ID NO. 6.