US 12,460,203 B2
CRISPR RNA targeting enzymes and systems and uses thereof
David R. Cheng, Boston, MA (US); David A. Scott, San Francisco, CA (US); Winston X. Yan, Brookline, MA (US); and Shaorong Chong, Arlington, MA (US)
Assigned to Arbor Biotechnologies, Inc., Cambridge, MA (US)
Filed by Arbor Biotechnologies, Inc., Cambridge, MA (US)
Filed on Nov. 8, 2021, as Appl. No. 17/521,617.
Application 17/521,617 is a division of application No. 15/916,274, filed on Mar. 8, 2018, granted, now 11,168,322.
Claims priority of provisional application 62/635,443, filed on Feb. 26, 2018.
Claims priority of provisional application 62/628,921, filed on Feb. 9, 2018.
Claims priority of provisional application 62/626,679, filed on Feb. 5, 2018.
Claims priority of provisional application 62/619,691, filed on Jan. 19, 2018.
Claims priority of provisional application 62/587,381, filed on Nov. 16, 2017.
Claims priority of provisional application 62/580,880, filed on Nov. 2, 2017.
Claims priority of provisional application 62/572,367, filed on Oct. 13, 2017.
Claims priority of provisional application 62/527,957, filed on Jun. 30, 2017.
Prior Publication US 2022/0127603 A1, Apr. 28, 2022
Int. Cl. C12N 15/11 (2006.01); C07K 14/195 (2006.01); C12N 9/22 (2006.01); C12N 15/113 (2010.01); C12N 15/62 (2006.01)
CPC C12N 15/11 (2013.01) [C07K 14/195 (2013.01); C12N 9/22 (2013.01); C12N 15/111 (2013.01); C12N 15/113 (2013.01); C12N 15/62 (2013.01); C12N 2310/20 (2017.05)] 21 Claims
 
1. An engineered, non-naturally occurring Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated (Cas) system comprising:
(a) an RNA guide or a nucleic acid encoding the RNA guide comprising a direct repeat sequence of SEQ ID NO: 65 and a spacer sequence capable of hybridizing to a target nucleic acid in a eukaryotic cell; and
(b) a nucleic acid encoding a CRISPR-Cas effector protein, or the CRISPR-Cas effector protein,
wherein the CRISPR-Cas effector protein binds to the RNA guide, and wherein the spacer sequence binds to a target nucleic acid.