	US 12,460,199 B2
	Modular polypeptide libraries and methods of making and using same
Wendell A. Lim, San Francisco, CA (US); Scott M. Coyle, San Francisco, CA (US); Russell M. Gordley, San Francisco, CA (US); and Kole T. Roybal, San Francisco, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Dec. 19, 2022, as Appl. No. 18/068,499.
Application 18/068,499 is a division of application No. 15/751,419, granted, now 11,560,561, previously published as PCT/US2016/049745, filed on Aug. 31, 2016.
Claims priority of provisional application 62/212,999, filed on Sep. 1, 2015.
Prior Publication US 2023/0212557 A1, Jul. 6, 2023
Int. Cl. C12N 15/10 (2006.01); A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/42 (2025.01); C07K 14/725 (2006.01); C07K 16/28 (2006.01)

CPC C12N 15/1079 (2013.01) [A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/4211 (2025.01); C07K 14/7051 (2013.01); C07K 16/2803 (2013.01); C12N 15/1037 (2013.01); C07K 2317/24 (2013.01); C07K 2319/02 (2013.01); C07K 2319/033 (2013.01); C07K 2319/30 (2013.01)]

10 Claims

1. A barcoded library of nucleic acids, wherein:

(i) the members of the library each comprise a barcode region and a coding sequence that is operably linked to a promoter;

(ii) within each member, the barcode region is separate from the coding sequence;

(iii) the coding sequence encodes a modular protein having at least a first domain, a second domain, and a third domain;

(iv) the sequences of the first domain, the second domain and the third domain independently vary in the library;

(v) the barcode region comprises at least a first barcode, a second barcode and a third barcode, and

(vi) within each member, the first barcode, the second barcode and the third barcode correspond to and identify the sequence of the first domain, the sequence of the second domain, and the sequence of the third domain of the modular protein, respectively.