	US 12,460,181 B2
	Membrane coverage and orientation and methods, compositions, and kits related thereto
Barney Bishop, Annandale, VA (US); and Mrinalini Ramanan, Fairfax, VA (US)
Assigned to GEORGE MASON UNIVERSITY, Fairfax, VA (US)
Filed by GEORGE MASON UNIVERSITY, Fairfax, VA (US)
Filed on May 12, 2020, as Appl. No. 16/872,639.
Claims priority of provisional application 62/847,982, filed on May 15, 2019.
Prior Publication US 2020/0362307 A1, Nov. 19, 2020
Int. Cl. A61K 9/00 (2006.01); A61K 47/69 (2017.01); A61K 49/00 (2006.01); C07D 271/12 (2006.01); C12N 5/00 (2006.01); C12N 5/078 (2010.01); C12N 15/88 (2006.01); G01N 21/64 (2006.01); G01N 33/50 (2006.01); G01N 33/543 (2006.01); G01N 33/554 (2006.01); G01N 33/58 (2006.01)

CPC C12N 5/0641 (2013.01) [A61K 47/6915 (2017.08); A61K 49/0084 (2013.01); C07D 271/12 (2013.01); C12N 5/0006 (2013.01); C12N 15/88 (2013.01); G01N 21/6428 (2013.01); G01N 33/5094 (2013.01); G01N 33/5432 (2013.01); G01N 33/554 (2013.01); G01N 33/586 (2013.01); G01N 2021/6432 (2013.01); G01N 2021/6439 (2013.01)]

5 Claims

1. A lipid-based structure comprising a lipid bilayer having a membrane portion derived from a plasma membrane of a cell and comprising a protein,

wherein the lipid bilayer isolates an interior space of the structure from an external environment,

wherein the protein of the membrane portion has the same orientation found on the plasma membrane of the cell with the protein,

wherein the protein has an extracellular domain covalently attached to a label, and

wherein the label is a fluorescent label.