	US 12,460,180 B2
	Methods for isolating and expanding cells
Shristi Bhandari, London (GB); Samuel Florence, London (GB); Andrew Hutton, London (GB); Louisa Mathias, London (GB); Oliver Nussbaumer, London (GB); Kalle Soderstrom, London (GB); and Mark Uden, London (GB)
Assigned to GammaDelta Therapeutics Limited, London (GB)
Appl. No. 17/292,411
Filed by GammaDelta Therapeutics Limited, London (GB)
PCT Filed Nov. 8, 2019, PCT No. PCT/GB2019/053166 § 371(c)(1), (2) Date May 7, 2021, PCT Pub. No. WO2020/095059, PCT Pub. Date May 14, 2020.
Claims priority of application No. 1818243 (GB), filed on Nov. 8, 2018.
Prior Publication US 2022/0010276 A1, Jan. 13, 2022
Int. Cl. C12N 5/0783 (2010.01); A61K 35/17 (2025.01); C12M 1/04 (2006.01)

CPC C12N 5/0638 (2013.01) [A61K 35/17 (2013.01); C12N 5/0646 (2013.01); C12N 2500/90 (2013.01); C12N 2501/2302 (2013.01); C12N 2501/2315 (2013.01)]

16 Claims

1. A method for the isolation of lymphocytes from a non-haematopoietic tissue sample comprising the steps of:

(i) placing the non-haematopoietic tissue sample in a vessel comprising a gas permeable silicone material, wherein the bottom of the vessel is configured to allow gas exchange from the bottom of the vessel;

(ii) culturing the non-haematopoietic tissue sample in the presence of Interleukin-2 (IL-2) and Interleukin-15 (IL-15); and

(iii) collecting a population of lymphocytes cultured from the non-haematopoietic tissue sample;

wherein the non-haematopoietic tissue sample is an intact biopsy obtained by punch biopsy from a non-haematopoietic tissue, and wherein the non-haematopoietic tissue sample has a minimum cross-section of at least 2 mm.