	US 12,460,177 B2
	Composition for promoting efficiency of somatic cell replication
Eun Ju Lee, Seoul (KR); and Hyo-Soo Kim, Seoul (KR)
Assigned to SEOUL NATIONAL UNIVERSITY HOSPITAL, Seoul (KR)
Appl. No. 16/969,045
Filed by SEOUL NATIONAL UNIVERSITY HOSPITAL, Seoul (KR)
PCT Filed Feb. 12, 2019, PCT No. PCT/KR2019/001686 § 371(c)(1), (2) Date Aug. 11, 2020, PCT Pub. No. WO2019/160299, PCT Pub. Date Aug. 22, 2019.
Claims priority of application No. 10-2018-0017472 (KR), filed on Feb. 13, 2018.
Prior Publication US 2021/0139845 A1, May 13, 2021
Int. Cl. C12N 5/073 (2010.01); C07K 14/575 (2006.01)

CPC C12N 5/0604 (2013.01) [C07K 14/57536 (2013.01); C12N 2501/998 (2013.01)]

4 Claims

1. A method of promoting somatic cell nuclear transfer efficiency, comprising:

a) contacting a donor cell with a composition comprising endothelin as an active ingredient, wherein the donor cell is a mesenchymal stem cell; and

b) extracting the nucleus of the donor cell, wherein the endothelin induces a transition of H3K9me3 to H3K4me3 in the Apobec1 promoter region of the extracted nucleus, thereby enhancing the efficiency of somatic cell nuclear transfer when the nucleus extracted in step b) thereby promoting blastocyst formation efficiency is transferred to an oocyte.