US 12,460,174 B2
Magnetic separation
Timothy Smith, Kingston (CA); Nuala Trainor, Kingston (CA); Kevin Middleton, Toronto (CA); and Alexis Pietak, Kingston (CA)
Assigned to OCTANE BIOTECH INC., Kingston (CA)
Appl. No. 17/279,353
Filed by OCTANE BIOTECH INC., Kingston (CA)
PCT Filed Sep. 25, 2019, PCT No. PCT/CA2019/051371
§ 371(c)(1), (2) Date Mar. 24, 2021,
PCT Pub. No. WO2020/061696, PCT Pub. Date Apr. 2, 2020.
Claims priority of provisional application 62/897,041, filed on Sep. 6, 2019.
Claims priority of provisional application 62/738,331, filed on Sep. 28, 2018.
Prior Publication US 2021/0403856 A1, Dec. 30, 2021
Int. Cl. B03C 1/02 (2006.01); C12M 1/00 (2006.01); C12M 1/36 (2006.01); C12N 5/0783 (2010.01)
CPC C12M 47/04 (2013.01) [B03C 1/02 (2013.01); C12M 41/48 (2013.01); C12N 5/0636 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A system for magnetically separating and collecting a target biological population from a biological sample, comprising:
a. a magnetic field source including multiple magnets;
b. a cell proliferation chamber;
c. one or more fluidics pathways;
d. a separation tube for flowing the biological sample, the separation tube running along a length of the multiple magnets, the separation tube being aligned with the magnetic field source;
e. a magnetic field shield/barrier configured to be rotated between the magnetic field source and the separation tube to release the target biological population bound to magnetic particles;
f. an electro-mechanical drive assembly comprising a servo and a gear train for rotating the magnetic field shield/barrier between the magnetic field source and the separation tube, wherein the magnetic field source and the magnetic field shield/barrier are connected to the servo and the gear train, allowing for the servo and the gear train to rotate the magnetic field source and the magnetic field shield/barrier; and
g. input locations for reagents;
wherein the magnetic field shield/barrier is rotated using the servo and the gear train such that the magnetic field shield/barrier is between the magnetic field source and the separation tube to release the target biological population when bound to the magnetic particles as the biological sample passes through the separation tube.