US 12,460,173 B2
Control of cell growth through a temperature feedback loop
Kathryn L. Aron, Acton, MA (US); and Jeffrey Swanberg, Maynard, MA (US)
Assigned to Bristol-Myers Squibb Company, Princeton, NJ (US)
Filed by Bristol-Myers Squibb Company, Princeton, NJ (US)
Filed on Nov. 7, 2022, as Appl. No. 18/053,192.
Application 18/053,192 is a continuation of application No. 16/367,837, filed on Mar. 28, 2019, abandoned.
Claims priority of provisional application 62/650,430, filed on Mar. 30, 2018.
Prior Publication US 2023/0090163 A1, Mar. 23, 2023
Int. Cl. C12M 1/36 (2006.01); C12M 1/34 (2006.01)
CPC C12M 41/48 (2013.01) [C12M 41/12 (2013.01); C12M 41/46 (2013.01)] 24 Claims
 
1. A method of controlling the growth of cells in a bioreactor comprising:
a) measuring the viable cell density of the cells growing in culture using a biomass capacitance probe; and
b) utilizing a temperature control loop to adjust the growth rate of the cells, wherein the temperature control loop utilizes a pre-determined growth curve and the measurement of the viable cell density in step a), wherein the biomass capacitance probe sends the measurement of the viable cell density in step a) to a control system, and wherein the control system continuously adjusts the temperature in the culture, wherein daily temperature oscillations in the reactor have a peak to peak amplitude of about 5° C. or less.