US 12,460,170 B2
Methods and systems for performing perfusion cell culture
Jochen Sieck, Darmstadt (DE); and Christian Schultheiss, Darmstadt (DE)
Assigned to MERCK PATENT GMBH, Darmstadt (DE)
Appl. No. 17/770,451
Filed by MERCK PATENT GMBH, Darmstadt (DE)
PCT Filed Nov. 5, 2020, PCT No. PCT/EP2020/081033
§ 371(c)(1), (2) Date Apr. 20, 2022,
PCT Pub. No. WO2021/089661, PCT Pub. Date May 14, 2021.
Claims priority of application No. 19207666 (EP), filed on Nov. 7, 2019.
Prior Publication US 2022/0380712 A1, Dec. 1, 2022
Int. Cl. C12M 1/00 (2006.01); C12M 1/26 (2006.01); C12M 1/34 (2006.01)
CPC C12M 29/10 (2013.01) [C12M 33/22 (2013.01); C12M 41/30 (2013.01); C12M 41/44 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A bioreactor system comprising:
(a) a bioreactor with a perfusion inlet for fresh media; (b) a cell retention device that separates media and cells, fluidly connected to the bioreactor via a harvest outlet for delivering harvest from the bioreactor to the cell retention device, the cell retention device directs cell free media for collection and retains cells in the bioreactor; and (c) a bleed recovery device comprising a means for separating cells from liquid, fluidly connected to the bioreactor via an inlet that delivers bleed from the bioreactor to the bleed recovery device, and an outlet for directing the liquid from the bleed to either the bioreactor or for collection.