US 12,460,168 B2
Packed-bed bioreactor systems and methods of using the same
Ann MeeJin Ferrie, Salem, NH (US); Vasiliy Nikolaevich Goral, Painted Post, NY (US); and Lori Eileen Romeo, Elmira Heights, NY (US)
Assigned to CORNING INCORPORATED, Corning, NY (US)
Filed by CORNING INCORPORATED, Corning, NY (US)
Filed on Jun. 18, 2024, as Appl. No. 18/746,891.
Application 18/206,501 is a division of application No. 16/781,807, filed on Feb. 4, 2020, granted, now 11,692,161, issued on Jul. 4, 2023.
Application 18/746,891 is a continuation of application No. 18/206,501, filed on Jun. 6, 2023, granted, now 12,037,572.
Claims priority of provisional application 62/910,696, filed on Oct. 4, 2019.
Claims priority of provisional application 62/801,325, filed on Feb. 5, 2019.
Prior Publication US 2024/0336883 A1, Oct. 10, 2024
Int. Cl. C12M 1/12 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12M 3/00 (2006.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01)
CPC C12M 25/14 (2013.01) [C12M 23/02 (2013.01); C12M 23/20 (2013.01); C12M 23/44 (2013.01); C12M 25/04 (2013.01); C12M 29/10 (2013.01); C12M 29/26 (2013.01); C12M 41/26 (2013.01); C12M 25/02 (2013.01); C12N 5/0068 (2013.01); C12N 5/0602 (2013.01); C12N 2513/00 (2013.01); C12N 2533/30 (2013.01); C12N 2533/40 (2013.01); C12N 2533/90 (2013.01); C12N 2535/00 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of culturing cells in a bioreactor system the method comprising:
providing a bioreactor system comprising:
a cell culture vessel comprising a first end, a second end, and at least one reservoir between the first and second ends; and
a cell culture matrix disposed in the at least one reservoir, the cell culture matrix comprising a plurality of woven substrates each comprising a plurality of interwoven fibers with surfaces configured for adhering cells thereto,
wherein the bioreactor system is configured to flow material through the at least one reservoir in a flow direction from the first end to the second end, and
wherein the substrates of the plurality of woven substrates are stacked such that each woven substrate is substantially perpendicular to the flow direction
seeding cells on the cell culture matrix;
culturing the cells on the cell culture matrix; and
harvesting a product of the culturing of the cells,
wherein the plurality of openings in the substrate is configured to allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate.