US 12,460,163 B2
Measurement cell manufacturing method and measurement cell
Yuzuru Shimazaki, Tokyo (JP); Tatsuo Nakagawa, Tokyo (JP); Junko Tanaka, Tokyo (JP); Akiko Shiratori, Tokyo (JP); and Chihiro Uematsu, Tokyo (JP)
Assigned to HITACHI, LTD.
Appl. No. 17/603,304
Filed by Hitachi, Ltd., Tokyo (JP)
PCT Filed Apr. 2, 2020, PCT No. PCT/JP2020/015220
§ 371(c)(1), (2) Date Oct. 12, 2021,
PCT Pub. No. WO2020/217922, PCT Pub. Date Oct. 29, 2020.
Claims priority of application No. 2019-081572 (JP), filed on Apr. 23, 2019.
Prior Publication US 2022/0195356 A1, Jun. 23, 2022
Int. Cl. C12M 1/34 (2006.01); G01N 21/03 (2006.01); G01N 35/08 (2006.01); G01N 37/00 (2006.01)
CPC C12M 1/34 (2013.01) [G01N 21/03 (2013.01); G01N 35/08 (2013.01); G01N 37/00 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A manufacturing method for manufacturing a measurement cell containing a liquid specimen,
the measurement cell including
a through-hole chip having a through-hole through which a liquid is introduced by capillary force,
a lower surface substrate placed to face the through-hole chip via a lower surface side space, and
an upper surface substrate placed to face the through-hole chip via an upper surface side space,
the lower surface substrate having a channel wall protruding from the lower surface substrate toward the through-hole chip to constitute a channel that guides a flow of liquid in the lower surface side space,
the manufacturing method comprising:
an introduction step of introducing the specimen into the through-hole by introducing a solution containing the specimen into the lower surface side space;
a step of discharging the solution remaining in the lower surface side space from the lower surface side space;
a step of introducing, into the lower surface side space, an anti-drying liquid that prevents drying of the specimen introduced into the through-hole; and
a step of introducing, into the upper surface side space, an anti-drying liquid that prevents drying of the specimen introduced into the through-hole,
wherein the channel has a shape that guides a flow of the solution from an inlet for introducing the solution into the lower surface side space toward an outlet for discharging the solution from the lower surface side space,
wherein the channel has a trench shape that guides the flow of the solution by extending along a direction in which the flow of the solution is generated between the inlet and the outlet, and
wherein the lower surface substrate includes a plurality of the channels each having the trench shape by including a plurality of the channel walls extending along the direction.
 
9. A measurement cell containing a liquid specimen, the measurement cell comprising:
a through-hole chip having a through-hole through which a liquid is introduced by capillary force;
a lower surface substrate placed to face the through-hole chip via a lower surface side space; and
an upper surface substrate placed to face the through-hole chip via an upper surface side space,
wherein
the lower surface substrate has a channel wall protruding from the lower surface substrate toward the through-hole chip to constitute a channel that guides a flow of liquid in the lower surface side space,
the through-hole contains the specimen,
the lower surface side space contains an anti-drying liquid that prevents drying of the specimen contained in the through-hole,
the upper surface side space contains an anti-drying liquid that prevents drying of the specimen contained in the through-hole,
the channel has a shape that guides a flow of a solution from an inlet for introducing the solution into the lower surface side space toward an outlet for discharging the solution from the lower surface side space,
the channel has a trench shape that guides the flow of the solution by extending along a direction in which the flow of the solution is generated between the inlet and the outlet, and
the lower surface substrate includes a plurality of the channels each having the trench shape by including a plurality of the channel walls extending along the direction.