	US 12,459,981 B2
	Primary NK CAR constructs and methods
Fereshteh Parviz, San Diego, CA (US); and Rohit Duggal, San Diego, CA (US)
Assigned to ImmunityBio, Inc., San Diego, CA (US)
Appl. No. 17/311,226
Filed by ImmunityBio, Inc., San Diego, CA (US)
PCT Filed Nov. 26, 2019, PCT No. PCT/US2019/063454 § 371(c)(1), (2) Date Jun. 4, 2021, PCT Pub. No. WO2021/107940, PCT Pub. Date Jun. 3, 2021.
Prior Publication US 2022/0282216 A1, Sep. 8, 2022
Int. Cl. C07K 14/55 (2006.01); A61K 40/15 (2025.01); A61K 40/31 (2025.01); A61K 40/42 (2025.01); A61K 45/06 (2006.01); C07K 14/705 (2006.01); C07K 14/725 (2006.01); C07K 16/28 (2006.01); C12N 5/0783 (2010.01); C12N 15/85 (2006.01)

CPC C07K 14/55 (2013.01) [A61K 40/15 (2025.01); A61K 40/31 (2025.01); A61K 40/421 (2025.01); A61K 40/4234 (2025.01); A61K 45/06 (2013.01); C07K 14/7051 (2013.01); C07K 14/70521 (2013.01); C07K 16/2827 (2013.01); C12N 5/0638 (2013.01); C12N 5/0646 (2013.01); C12N 15/85 (2013.01); C07K 2317/622 (2013.01); C07K 2317/732 (2013.01); C07K 2319/03 (2013.01); C07K 2319/33 (2013.01); C12N 2510/00 (2013.01); C12N 2830/001 (2013.01); C12N 2830/50 (2013.01); C12N 2840/203 (2013.01)]

4 Claims

1. A recombinant nucleic acid, comprising:

a T7 promoter sequence portion, a 5′ untranslated (5′-UTR) sequence portion, a sequence portion encoding a signal peptide, a sequence portion encoding a single chain antibody fragment, a sequence portion encoding a hinge region, a sequence portion encoding a transmembrane domain, and a sequence portion encoding one or more intracellular domains; and

wherein the nucleic acid comprises any one of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4.