US 12,459,972 B2
Protease-activated contrast agents for in vivo imaging
Matthew S. Bogyo, Redwood City, CA (US); Martijn Verdoes, Nijmegen (NL); Leslie Ofori, Jupiter, FL (US); and Nimali P. Withana, Menlo Park, CA (US)
Assigned to The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed by THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY, Palo Alto, CA (US)
Filed on Nov. 8, 2022, as Appl. No. 18/053,682.
Application 16/792,142 is a division of application No. 15/545,689, granted, now 10,570,173, issued on Feb. 25, 2020, previously published as PCT/US2016/014606, filed on Jan. 22, 2016.
Application 18/053,682 is a continuation of application No. 16/792,142, filed on Feb. 14, 2020, abandoned.
Claims priority of provisional application 62/106,400, filed on Jan. 22, 2015.
Prior Publication US 2024/0025945 A1, Jan. 25, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 5/068 (2006.01); A61K 49/00 (2006.01); A61K 51/08 (2006.01); C07D 401/14 (2006.01); C07D 403/08 (2006.01); C07D 405/06 (2006.01)
CPC C07K 5/06086 (2013.01) [A61K 49/0032 (2013.01); A61K 49/0056 (2013.01); A61K 51/08 (2013.01); A61K 51/088 (2013.01); C07D 401/14 (2013.01); C07D 403/08 (2013.01); C07D 405/06 (2013.01)] 19 Claims
 
1. A method of labeling a tissue in an animal comprising the step of:
administering a composition to the animal, wherein the composition comprises:
a compound and a pharmaceutically acceptable carrier;
wherein the compound has a formula (I)

OG Complex Work Unit Chemistry
wherein D is a detectable element comprising a fluorescent label;
Q is a QSY quencher or QC-1;
L0 and L1 is each independently a C2-8 alkyl linker;
AA2 is an aralkyl amino acid side chain;
U is O;
R is an aralkyl group.