	US 12,459,971 B2
	Albumin rich pea protein isolate and process for production thereof
Prasanth Kumar Sasidharan Pillai, Livermore, CA (US); and Manoj Kumar, Livermore, CA (US)
Assigned to Louis Dreyfus Company Plant Proteins LLP, Livermore, CA (US)
Filed by Louis Dreyfus Company Plant Proteins LLC, Livermore, CA (US)
Filed on Apr. 18, 2025, as Appl. No. 19/183,742.
Claims priority of provisional application 63/636,428, filed on Apr. 19, 2024.
Prior Publication US 2025/0326788 A1, Oct. 23, 2025
Int. Cl. C07K 1/30 (2006.01)

CPC C07K 1/303 (2013.01)

7 Claims

1. A method of making an albumin enriched pea protein isolate consisting essentially of:

a) performing a protein extraction of pea flour with an aqueous solution,

i) at a pH of about 7 to 10,

ii) at a temperature of about 18° C. to 60° C., and

iii) for about 30 minutes to 60 minutes for the protein extraction;

b) separating solids from the aqueous solution in step a) to produce a liquid fraction;

c) precipitation of protein from the liquid fraction by

i) precipitating protein at a pH about 4 to 8, at a temperature of about 18° C. to 95° C. for about 5 to 60 minutes, and

ii) using 0.1% to 5% sodium hexametaphosphate to produce the albumin enriched pea protein isolate.