US 12,459,842 B2
Method for producing microbial cellular biomass having flocculant properties
Yolaine Bessiere, Toulouse (FR); Etienne Paul, Toulouse (FR); Jean-Noel Louvet, Toulouse (FR); Elise Blanchet, Toulouse (FR); Evrard Mengelle, Toulouse (FR); Simon Dubos, Toulouse (FR); Mansour Bounouba, Toulouse (FR); Carine Saux, Toulouse (FR); Ana Morgado Ferreira, Toulouse (FR); and Dores Cirne, Saint-Maurice (FR)
Assigned to INSTITUT NATIONAL DES SCIENCES APPLIQUÉES DE TOULOUSE, Toulouse (FR); CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR); and INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT, Paris (FR)
Appl. No. 17/434,794
Filed by Institut National des Sciences Appliquées de Toulouse, Toulouse (FR); Centre National de la Recherche Scientifique, Paris (FR); and Institut national de recherche pour l'agriculture, l'alimentation et l'environnement, Paris (FR)
PCT Filed Mar. 10, 2020, PCT No. PCT/FR2020/050489
§ 371(c)(1), (2) Date Mar. 23, 2022,
PCT Pub. No. WO2020/183103, PCT Pub. Date Sep. 17, 2020.
Claims priority of application No. 1902451 (FR), filed on Mar. 11, 2019.
Prior Publication US 2022/0242759 A1, Aug. 4, 2022
Int. Cl. C02F 1/32 (2023.01); C02F 1/52 (2023.01); C02F 3/34 (2023.01); C12N 1/20 (2006.01); C12P 19/04 (2006.01)
CPC C02F 1/5263 (2013.01) [C02F 3/34 (2013.01); C12N 1/20 (2013.01); C12P 19/04 (2013.01); C02F 2305/06 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A process for producing a microbial cellular biomass having flocculant properties comprising the steps:
a) introducing into a reactor a substrate comprising at least 30% by weight of monosaccharides to form a reaction medium,
wherein the reaction medium has a C/N molar ratio of 1 to 9.5, a C/P molar ratio of 40 to less than or equal to 600, and a dilution rate D of 0.05 to less than or equal to 0.35 h−1;
b) stirring the reaction medium obtained in step a) while retaining the C/N molar ratio at a value of 1 to 9.5, the C/P molar ratio at a value of 40 to less than or equal to 600, and the dilution rate D at a value of 0.05 to less than or equal to 0.35 h−1;
wherein the C/N molar ratios of steps a) and b) are identical, and the C/P molar ratios of steps a) and b) are identical; and
c) eliminating any filamentous bacteria and filamentous fungi by sequential decantation of the reaction medium obtained in step b) to obtain a decanted solid and also the microbial cellular biomass having flocculant properties.