US 12,458,970 B2
Light-digital PCR chamber and light-digital PCR device using same
Oh Seok Kwon, Daejeon (KR); Kyung Ho Kim, Daejeon (KR); Seon Joo Park, Daejeon (KR); Seong Eun Seo, Daejeon (KR); and Tai Hwan Ha, Daejeon (KR)
Assigned to KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY, Daejeon (KR)
Appl. No. 17/291,180
Filed by KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY, Daejeon (KR)
PCT Filed Nov. 19, 2019, PCT No. PCT/KR2019/015897
§ 371(c)(1), (2) Date May 4, 2021,
PCT Pub. No. WO2020/106045, PCT Pub. Date May 28, 2020.
Claims priority of application No. 10-2018-0142944 (KR), filed on Nov. 19, 2018.
Prior Publication US 2022/0062888 A1, Mar. 3, 2022
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/508 (2013.01) [B01L 3/502715 (2013.01); B01L 2300/0861 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/12 (2013.01); B01L 2300/168 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A light-digital polymerase chain reaction (PCR) chamber comprising:
a transparent substrate configured to pass light;
a metal thin film layer placed on a top surface of the transparent substrate;
a light shielding layer placed on a top surface of the metal thin film layer and configured to prevent the light passing through the transparent substrate from reaching a microchannel structure layer; and
the microchannel structure layer placed on a top surface of the light shielding layer, the microchannel structure layer including a plurality of wells, the plurality of wells each configured to accommodate a sample to be detected and perform a PCR reaction therein,
wherein the sample within the wells of the microchannel structure layer does not directly contact with the light shielding layer,
wherein the light shielding layer is configured to prevent the light passing through the transparent substrate from reaching the microchannel structure layer,
wherein the light shielding layer is at least one selected from a polydopamine film, a photocatalyst organic compound film, and a dabcyl organic compound film, and
wherein the light is visible light or infrared light.