US 12,458,966 B2
Microfluidic array, method of manufacture, measuring system comprising the microfluidic array, and use
Sabine Ziemba, Nuremberg (DE); Michael Scharfenberg, Nuremberg (DE); Violetta Olszowka, Zirndorf (DE); Martina Schmidt, Nuremberg (DE); Juri Attner, Unterägeri (CH); Markus Bigler, Hochdorf (CH); Sascha Mario Epp, Steinhausen (CH); Martin Richner, Auw (CH); Maria Bauer, Salzburg (AT); Gottfried Reiter, Adnet (AT); and Georg Bauer, Salzburg (AT)
Assigned to LEONHARD KURZ Stiftung & Co. KG, Fürth (DE); and OVD Kinegram AG, Zug (CH)
Appl. No. 17/610,306
Filed by LEONHARD KURZ Stiftung & Co. KG, Fürth (DE); OVD Kinegram AG, Zug (CH); and Stratec Consumables GmbH, Anif (AT)
PCT Filed Mar. 11, 2020, PCT No. PCT/EP2020/056465
§ 371(c)(1), (2) Date Nov. 10, 2021,
PCT Pub. No. WO2020/229016, PCT Pub. Date Nov. 19, 2020.
Claims priority of application No. 10 2019 112 254.6 (DE), filed on May 10, 2019; and application No. 10 2019 135 764.0 (DE), filed on Dec. 23, 2019.
Prior Publication US 2022/0241781 A1, Aug. 4, 2022
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/502707 (2013.01) [B01L 2300/0887 (2013.01); B01L 2300/123 (2013.01); B01L 2300/168 (2013.01); B01L 2400/0406 (2013.01)] 28 Claims
OG exemplary drawing
 
1. A method for producing a microfluidic array comprising at least one flow channel covered at least in regions, in fluid connection with at least one inlet and at least one outlet, wherein the method comprises the following steps:
a) providing at least one base ply,
b) providing at least one flexible cover ply, comprising at least one structural element, which is arranged on a surface of at least one side of the cover ply, and
c) arranging the at least one flexible cover ply on at least one partial region of the at least one base ply, with the result that at least one partial region of the at least one base ply is arranged on at least one partial region of the at least one structural element arranged on the surface of at least one side of the cover ply to form at least one flow channel covered at least in regions,
wherein the at least one flexible cover ply comprises a cover layer made of at least one replication varnish, wherein the at least one structural element is arranged on a surface of at least one side of the cover layer.
 
8. The method according to claim 1, wherein the at least one base ply has macroscopic structures with a minimum structure size of greater than 10 μm, the macroscopic structures being selected from the group consisting of inlet, outlet, through-hole, depression, bulge, wall element, channel element, pre-chamber, mixing chamber, collection chamber, analysis chamber or combinations thereof.
 
22. A method for producing a microfluidic array comprising at least one flow channel covered at least in regions, in fluid connection with at least one inlet and at least one outlet, wherein the method comprises the following steps:
a) providing at least one base ply,
b) providing at least one flexible cover ply, comprising at least one structural element, which is arranged on a surface of at least one side of the cover ply, and
c) arranging the at least one flexible cover ply on at least one partial region of the at least one base ply, with the result that at least one partial region of the at least one base ply is arranged on at least one partial region of the at least one structural element arranged on the surface of at least one side of the cover ply to form at least one flow channel covered at least in regions,
wherein at least one cover ply is provided in the form of a transfer film, wherein the transfer film furthermore has at least one first carrier ply, which is arranged detachably on the side of the at least one cover ply lying opposite the at least one structural element.
 
28. A method for producing a microfluidic array comprising at least one flow channel covered at least in regions, in fluid connection with at least one inlet and at least one outlet, wherein the method comprises the following steps:
a) providing at least one base ply,
b) providing at least one flexible cover ply, comprising at least one structural element, which is arranged on a surface of at least one side of the cover ply, and
c) arranging the at least one flexible cover ply on at least one partial region of the at least one base ply, with the result that at least one partial region of the at least one base ply is arranged on at least one partial region of the at least one structural element arranged on the surface of at least one side of the cover ply to form at least one flow channel covered at least in regions,
wherein the at least one cover ply is provided in the form of a transfer film, wherein the transfer film furthermore has at least one carrier ply, which is arranged non-detachably on the side of the at least one cover ply lying opposite the at least one structural element.