US 12,458,734 B2
Silk fibroin/hydroxyapatite composite material, and preparation method therefor and application thereof
Chunyu Chang, Hubei (CN); Lin Wu, Hubei (CN); Yu Pan, Hubei (CN); and Jiehan Lin, Hubei (CN)
Assigned to Hubei Sailuo Biological Material Co., Ltd, Wuhan (CN)
Appl. No. 18/282,535
Filed by Hubei Sailuo Biological Material Co., Ltd, Hubei (CN)
PCT Filed Mar. 9, 2022, PCT No. PCT/CN2022/079918
§ 371(c)(1), (2) Date Sep. 17, 2023,
PCT Pub. No. WO2022/242272, PCT Pub. Date Nov. 24, 2022.
Claims priority of application No. 202110553568.6 (CN), filed on May 20, 2021.
Prior Publication US 2024/0181138 A1, Jun. 6, 2024
Int. Cl. A61L 31/12 (2006.01); A61L 31/14 (2006.01)
CPC A61L 31/127 (2013.01) [A61L 31/148 (2013.01); A61L 2400/12 (2013.01); A61L 2430/02 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A preparation method of a silk fibroin/hydroxyapatite composite material, comprising:
(1) maintaining a uniform mixture of hydroxyapatite nanoparticles, silk fibroin, and hexafluoroisopropanol at a temperature range from 50° C. to 60° C. for at least 3 hours to obtain a viscous silk fibroin/hydroxyapatite solution;
(2) pouring the silk fibroin/hydroxyapatite solution into a long cylindrical mold; and two ends of the long cylindrical mold being respectively an end A and an end B;
(3) at room temperature, making the end A opened and the end B closed, vertically immersing the mold in methanol with the opening facing up and letting the mold stand for at least 2 days; and turning the mold upside down, making the end A closed and the end B opened, vertically immersing the mold in the methanol with the opening facing up and letting the mold stand for at least 2 days; and
(4) removing the mold, and performing ventilation and drying to obtain a silk fibroin/hydroxyapatite composite material.