US 12,458,731 B2
Mesenchymal cell-binding composite material for tissue restoration
Russell Martin, Baltimore, MD (US); Hai-quan Mao, Baltimore, MD (US); Sashank Reddy, Baltimore, MD (US); Justin Sacks, Baltimore, MD (US); Xiaowei Li, Towson, MD (US); and Brian Honewee Cho, Baltimore, MD (US)
Assigned to The Johns Hopkins University, Baltimore, MD (US)
Appl. No. 15/753,272
Filed by The Johns Hopkins University, Baltimore, MD (US)
PCT Filed Aug. 17, 2016, PCT No. PCT/US2016/047285
§ 371(c)(1), (2) Date Feb. 17, 2018,
PCT Pub. No. WO2017/031169, PCT Pub. Date Feb. 23, 2017.
Claims priority of provisional application 62/206,019, filed on Aug. 17, 2015.
Prior Publication US 2018/0243480 A1, Aug. 30, 2018
Int. Cl. A61L 27/52 (2006.01); A61K 35/28 (2015.01); A61K 38/08 (2019.01); A61K 39/395 (2006.01); A61L 27/36 (2006.01); A61L 27/38 (2006.01); A61L 27/48 (2006.01); A61L 27/54 (2006.01); A61L 27/56 (2006.01); A61L 27/58 (2006.01); A61L 27/60 (2006.01); C12N 5/0775 (2010.01)
CPC A61L 27/48 (2013.01) [A61K 35/28 (2013.01); A61K 38/08 (2013.01); A61K 39/395 (2013.01); A61L 27/3604 (2013.01); A61L 27/3608 (2013.01); A61L 27/38 (2013.01); A61L 27/3834 (2013.01); A61L 27/52 (2013.01); A61L 27/54 (2013.01); A61L 27/56 (2013.01); A61L 27/58 (2013.01); A61L 27/60 (2013.01); C12N 5/0667 (2013.01); A61L 2400/06 (2013.01); A61L 2430/34 (2013.01); C12N 2513/00 (2013.01); C12N 2533/30 (2013.01); C12N 2533/90 (2013.01)] 21 Claims
 
1. A soft tissue device, comprising:
a mesenchymal cell-binding moiety covalently linked to a scaffold complex, and comprising a plurality of pores present on or within a surface of the device, wherein the pores are present at a concentration of at least 50 pores per cm2 of the surface, and wherein at least 80% of the pores have an average pore diameter is at least 5 microns, wherein:
a. the mesenchymal cell-binding moiety comprises an amino acid sequence selected from among CMLAGWIPC (SEQ ID NO: 2), CWLGEWLGC (SEQ ID NO: 4), CSWKYWFGEC (SEQ ID NO: 5), CGQWLGNWLC (SEQ ID NO: 6) or CKGGRAKDC (SEQ ID NO: 7); and
b. the scaffold complex comprises a polymeric fiber having a mean diameter of from 100 nm to 8000 nm covalently linked to a hydrogel material, wherein the ratio of fiber to hydrogel material is from 1:10 to 10:1 on a component-mass basis, wherein:
i. the hydrogel material comprises a hyaluronic acid, or a derivative thereof; and
ii. the polymeric fiber comprises a biocompatible biodegradable polyester, wherein the polymeric fiber comprises maleimide groups and the surface density of the maleimide groups on the polymeric fiber is from 10 nmol/mg of fibers to 160 nmol/mg of the fiber.