US 12,458,708 B2
Marine polysaccharide vector-based anthocyanin nanoparticles, and preparation method therefor and application thereof in targeted delivery
Mingqian Tan, Dalian (CN); Xue Zhao, Dalian (CN); Xuedi Zhang, Dalian (CN); Shanshan Tie, Dalian (CN); Jiyun Cao, Dalian (CN); and Yun Li, Dalian (CN)
Assigned to Dalian Polytechnic University, Dalian (CN)
Filed by Dalian Polytechnic University, Dalian (CN)
Filed on Jun. 1, 2023, as Appl. No. 18/204,436.
Application 18/204,436 is a continuation of application No. PCT/CN2020/142199, filed on Dec. 31, 2020.
Claims priority of application No. 202011385600.6 (CN), filed on Dec. 2, 2020.
Prior Publication US 2023/0355802 A1, Nov. 9, 2023
Int. Cl. A61K 47/69 (2017.01); A61K 9/19 (2006.01); A61K 31/352 (2006.01); A61K 49/00 (2006.01); A61P 1/00 (2006.01); A61P 39/06 (2006.01)
CPC A61K 47/6939 (2017.08) [A61K 9/19 (2013.01); A61K 31/352 (2013.01); A61K 49/0054 (2013.01); A61K 49/0093 (2013.01); A61P 1/00 (2018.01); A61P 39/06 (2018.01)] 8 Claims
 
1. Marine polysaccharide vector-based anthocyanin nanoparticles, wherein the anthocyanin nanoparticles comprises sodium alginate, food-borne fluorescent nanoparticles and sodium hyaluronate as a composite vector combined with anthocyanin.
 
2. A preparation method for the marine polysaccharide vector-based anthocyanin nanoparticles according to claim 1, comprising the following steps:
S1: dissolving sodium alginate in phosphate buffered saline with a pH value of 4.75-6, adding 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC) and 1-hydroxybenzotriazole (HOBt) successively, stirring at room temperature for 60-80 minutes and then adding food-borne fluorescent nanoparticles, continuing to stir for 24-30 hours, and carrying out dialysis with deionized water for 24-72 hours and freeze drying to obtain a fluorescence-alginate complex;
S2: adding the fluorescence-alginate complex into N, N-dimethylformamide (DMF) and (dichloromethane (DCM) which are mixed in a volume ratio of 1:(1-1.5), adding diisopropyl carbodiimide (DIC) and dimethylaminopyridine (DMAP) thereinto, and stirring at room temperature for 1-1.5 hours;
S3: adding anthocyanin and hyaluronic acid to react at room temperature for 24-30 hours, and washing with the DCM for 3-5 times to remove a solvent; and
S4: removing unreacted small molecules and the DMF, pre-cooling at −20° C. to −80° C. for 2-3 hours, and carrying out freeze drying at −50° C. to −55° C. for 24-72 hours under the condition that the vacuum degree is 35-45 Pa.