	US 12,458,698 B2
	Expansion of γδ T cells, compositions, and methods of use thereof
Richard Beatson, London (GB); Adrian Hayday, Kent (GB); Oliver Nussbaumer, London (GB); Richard Woolf, London (GB); Maria Luisa Iannitto, London (GB); and Natalie Mount, London (GB)
Assigned to King's College London, London (GB); and GammaDelta Therapeutics Limited, London (GB)
Filed by King's College London, London (GB); and GammaDelta Therapeutics Limited, London (GB)
Filed on Jan. 12, 2023, as Appl. No. 18/153,900.
Application 18/153,900 is a continuation of application No. 16/610,398, granted, now 11,655,453, previously published as PCT/EP2018/061413, filed on May 3, 2018.
Claims priority of application No. 1707048 (GB), filed on May 3, 2017.
Prior Publication US 2023/0242878 A1, Aug. 3, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/0783 (2010.01); A61K 40/11 (2025.01); A61K 40/42 (2025.01); A61K 45/06 (2006.01)

CPC A61K 45/06 (2013.01) [A61K 40/11 (2025.01); A61K 40/42 (2025.01); C12N 5/0636 (2013.01); C12N 5/0638 (2013.01); C12N 2500/90 (2013.01); C12N 2501/21 (2013.01); C12N 2501/2301 (2013.01); C12N 2501/2302 (2013.01); C12N 2501/2304 (2013.01); C12N 2501/2309 (2013.01); C12N 2501/2312 (2013.01); C12N 2501/2315 (2013.01); C12N 2501/2318 (2013.01); C12N 2501/2321 (2013.01); C12N 2501/2333 (2013.01)]

20 Claims

1. An expanded population of γδ T cells obtained by a method comprising the steps of:

(i) providing a population of γδ T cells obtained from a non-haematopoietic tissue, wherein the non-haematopoietic tissue is skin or gut; and

(ii) culturing the population of γδ T cells in the presence of:

a. I1-2 or IL-9;

b. IL-15; and

c. IL-21;

for at least 5 days in amounts effective to produce an expanded population of γδ T cells.