US 12,458,596 B2
Immunomodulatory hydrogel and preparation method thereof
Huijuan Song, Tianjin (CN); Qiang Liu, Tianjin (CN); Ningning He, Tianjin (CN); Manman Zhang, Tianjin (CN); Yan Wang, Tianjin (CN); Jinhan Wang, Tianjin (CN); Liqing Du, Tianjin (CN); Chang Xu, Tianjin (CN); and Kaihua Ji, Tianjin (CN)
Assigned to Institute of Radiation Medicine, Chinese Academy of Medical Selences & Peking Union Medical College, Tianjin (CN)
Filed by Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin (CN)
Filed on Dec. 30, 2024, as Appl. No. 19/004,480.
Claims priority of application No. 202311687739.X (CN), filed on Jan. 3, 2024.
Prior Publication US 2025/0213472 A1, Jul. 3, 2025
Int. Cl. A61K 9/107 (2006.01); A61K 9/06 (2006.01); A61K 31/194 (2006.01); A61K 47/34 (2017.01); A61P 29/00 (2006.01); A61P 37/02 (2006.01)
CPC A61K 9/1075 (2013.01) [A61K 9/06 (2013.01); A61K 31/194 (2013.01); A61K 47/34 (2013.01); A61P 29/00 (2018.01); A61P 37/02 (2018.01)] 11 Claims
 
1. An immunomodulatory hydrogel, wherein methoxy polyethylene glycol amine (mPEG-NH2) is used as an initiator to react with L-tyrosine-N-carboxyanhydrides (L-Tyr-NCAs) to form a methoxy polyethylene glycol-poly L-tyrosine (PETyr) as a PETyr block copolymer, and a structural formula of the PETyr is

OG Complex Work Unit Chemistry
the PETyr reacts with tyrosinase to form an oxidized methoxy polyethylene glycol-poly L-tyrosine copolymer (PETO), and the PETO is co-dissolved with octyl itaconate nanomicelles (ITA8) in a disperse medium to obtain an itaconic acid-loaded immunomodulatory hydrogel (PETO-ITA8); and
wherein a concentration of the PETyr in a hydrogel system is 50 milligrams per milliliter (mg/mL) to 300 mg/mL, and a concentration of the ITA8 in the hydrogel system is 62.5 micromoles per liter (μM) to 1 millimole per liter (mM).