US 12,457,951 B2
Alteration of flavor traits in consumer crops via disablement of the myrosinase/glucosinolate system
Dale Karlson, Cary, NC (US); HaeJin Kim, Durham, NC (US); Thomas J. Poorten, Durham, NC (US); and Julius Mojica, Durham, NC (US)
Assigned to Pairwise Plants Services, Inc., Durham, NC (US)
Appl. No. 17/634,694
Filed by PAIRWISE PLANTS SERVICES, INC., Durham, NC (US)
PCT Filed Aug. 14, 2020, PCT No. PCT/US2020/046483
§ 371(c)(1), (2) Date Feb. 11, 2022,
PCT Pub. No. WO2021/030738, PCT Pub. Date Feb. 18, 2021.
Claims priority of provisional application 62/886,458, filed on Aug. 14, 2019.
Prior Publication US 2022/0377995 A1, Dec. 1, 2022
Int. Cl. A01H 1/00 (2006.01); A01H 6/20 (2018.01); C12N 15/82 (2006.01)
CPC A01H 1/101 (2021.01) [A01H 6/20 (2018.05); C12N 15/8201 (2013.01); C12N 2310/20 (2017.05)] 8 Claims
 
1. A Brassica juncea plant or part thereof comprising at least one mutation in an endogenous gene encoding a myrosinase, wherein the plant or part thereof comprising at least one mutation in an endogenous gene exhibits a reduction in the activity of the myrosinase, wherein the endogenous gene encoding the myrosinase comprises a nucleotide sequence encoding a polypeptide having at least 80% sequence identity to any one of the amino acid sequences of SEQ ID NOs: 93-105.