US 12,130,292 B2
Full length kinase activity-conformation reporter
Eduard Stefan, Götzens (AT); and Johanna Mayrhofer, Innsbruck (AT)
Assigned to KINCON BIOLABS GMBH, Innsbruck (AT)
Filed by KINCON BIOLABS GMBH, Innsbruck (AT)
Filed on Dec. 20, 2021, as Appl. No. 17/556,437.
Application 17/556,437 is a continuation of application No. 16/337,217, granted, now 11,237,173, previously published as PCT/EP2017/074761, filed on Sep. 29, 2017.
Claims priority of application No. 16191530 (EP), filed on Sep. 29, 2016.
Prior Publication US 2022/0137060 A1, May 5, 2022
Int. Cl. G01N 33/68 (2006.01)
CPC G01N 33/6845 (2013.01) [G01N 2333/71 (2013.01); G01N 2500/10 (2013.01)] 27 Claims
 
1. A polynucleotide encoding for a reporter for a protein fragment complementation assay:
wherein the reporter is a single fused protein comprising a first fragment, a second fragment and a protein kinase sequence section,
wherein the first fragment and the second fragment are derived from different sections of a split protein, and
wherein the protein kinase sequence section intervenes between the first fragment and the second fragment and
wherein the protein kinase sequence section comprises a catalytic kinase domain sequence and one or more regulatory sequence(s).