US 12,130,285 B2
Apoptotic cell mimic
Keunho Ahn, Pleasanton, CA (US); Zhenyu Deng, Berkeley, CA (US); Martina De Geus, Berkeley, CA (US); and Anh Tuan Nguyen, San Francisco, CA (US)
Assigned to Slingshot Biosciences, Inc., Emeryville, CA (US)
Filed by Slingshot Biosciences, Inc., Emeryville, CA (US)
Filed on Mar. 18, 2024, as Appl. No. 18/608,025.
Application 18/608,025 is a continuation of application No. PCT/US2023/067893, filed on Jun. 2, 2023.
Claims priority of provisional application 63/348,414, filed on Jun. 2, 2022.
Prior Publication US 2024/0219382 A1, Jul. 4, 2024
Int. Cl. G01N 33/543 (2006.01); G01N 15/10 (2024.01); G01N 15/14 (2024.01)
CPC G01N 33/5436 (2013.01) [G01N 15/1012 (2013.01); G01N 15/14 (2013.01); G01N 2015/1006 (2013.01)] 25 Claims
OG exemplary drawing
 
1. A kit comprising: a) a first population of hydrogel beads, each hydrogel bead comprising: i) a polymerized monomer; ii) annexin V; and iii) an encapsulated nucleic acid; b) a second population of hydrogel beads, each hydrogel bead comprising: i) a polymerized monomer; ii) annexin V; but iii) lacking the encapsulated nucleic acid of the first population of hydrogel beads; and c) a third population of hydrogel beads, each hydrogel bead comprising: i) a polymerized monomer; but ii) lacking the annexin V of the first population of hydrogel beads and the second population of hydrogel beads; and iii) lacking the encapsulated nucleic acid of the first population of hydrogel beads; wherein the annexin V of the first and/or second population of hydrogel beads comprises SEQ ID NO: 102, and wherein the encapsulated nucleic acid of the first population of hydrogel beads binds to an intercalating dye selected from the group consisting of 7-aminoactinomycin D (7AAD) and propidium iodide.