	US 12,129,506 B2
	Microorganisms and methods for the production of fatty acids and fatty acid derived products
Michael D. Lynch, Durham, NC (US); Michael Tai Man Louie, Plymouth, MN (US); Shelley Copley, Boulder, CO (US); Eileen Colie Spindler, Lafayette, CO (US); Brittany Robinson, Wheat Ridge, CO (US); Matthew Lipscomb, Boulder, CO (US); Tanya Lipscomb-Warnecke, Boulder, CO (US); Hans H. Liao, Plymouth, MN (US); David Hogsett, Niwot, CO (US); and Ron Evans, Louisville, CO (US)
Assigned to CARGILL, INCORPORATED, Wayzata, MN (US)
Filed by CARGILL, INCORPORATED, Wayzata, MN (US)
Filed on Jun. 29, 2022, as Appl. No. 17/809,750.
Application 17/809,750 is a continuation of application No. 16/453,323, filed on Jun. 26, 2019, granted, now 11,408,013.
Application 16/453,323 is a continuation of application No. 14/904,862, granted, now 10,337,038, issued on Jul. 2, 2019, previously published as PCT/US2014/047320, filed on Jul. 18, 2014.
Claims priority of provisional application 61/856,652, filed on Jul. 19, 2013.
Prior Publication US 2023/0033275 A1, Feb. 2, 2023
Int. Cl. C12P 7/6409 (2022.01); C12N 1/20 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01)

CPC C12P 7/6409 (2013.01) [C12N 1/20 (2013.01); C12N 9/0006 (2013.01); C12N 9/001 (2013.01); C12N 9/1029 (2013.01); C12N 9/88 (2013.01); C12Y 101/01035 (2013.01); C12Y 103/01093 (2015.07); C12Y 203/01194 (2013.01); C12Y 402/01059 (2013.01); C12Y 203/01075 (2013.01); C12Y 301/02 (2013.01)]

19 Claims

1. A genetically modified organism comprising a heterologous nucleic acid sequence encoding a NphT7 polypeptide, a heterologous nucleic acid sequence encoding a thioesterase, and comprising a heterologous nucleic acid sequence encoding a modified NphT7 polypeptide; wherein the modified NphT7 polypeptide comprises amino acid substitutions at positions Ile147 and Phe217, and wherein said microorganism is capable of producing an acyl-CoA having a carbon chain length of C6 or greater.