	US 12,129,488 B2
	Production of brown adipocytes
Marine Kraus, Vuarrens (CH); Stephen Dalton, Athens, GA (US); and John W. Avery, III, Snellville, GA (US)
Assigned to Societe des Produits Nestle S.A., Vevey (CH)
Appl. No. 17/044,085
Filed by SOCIETE DES PRODUITS NESTLE S.A., Vevey (CH)
PCT Filed Apr. 16, 2019, PCT No. PCT/EP2019/059724 § 371(c)(1), (2) Date Sep. 30, 2020, PCT Pub. No. WO2019/228705, PCT Pub. Date Dec. 5, 2019.
Claims priority of application No. 18174484 (EP), filed on May 28, 2018.
Prior Publication US 2021/0139853 A1, May 13, 2021
Int. Cl. C12N 5/071 (2010.01); A61K 35/35 (2015.01); A61P 3/10 (2006.01); C12N 5/077 (2010.01)

CPC C12N 5/0653 (2013.01) [A61P 3/10 (2018.01); C12N 2501/999 (2013.01)]

10 Claims

1. A method for in vitro differentiation of human induced pluripotent stem (hIPSC) cells into a population of brown adipocytes, the method comprising culturing the hIPSC cells for about 1-15 days with a transforming growth factor beta (TGF-β) inhibitor to obtain a first population of cells, and then culturing the first population of cells for about 20-50 days in the absence of the TGF-β inhibitor to obtain a second population of cells; and then culturing the second population of cells to form aggregated brown adipocytes.