	US 12,129,483 B2
	Compositions and methods for making sensory neurons
Nadja Zeltner, Athens, GA (US); and Vicente Kenyi Saito-Diaz, Athens, GA (US)
Assigned to UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC., Athens, GA (US)
Filed by University of Georgia Research Foundation, Inc., Athens, GA (US)
Filed on Dec. 20, 2021, as Appl. No. 17/555,581.
Claims priority of provisional application 63/127,958, filed on Dec. 18, 2020.
Prior Publication US 2022/0195386 A1, Jun. 23, 2022
Int. Cl. C12N 5/0793 (2010.01); A61K 35/30 (2015.01)

CPC C12N 5/0619 (2013.01) [A61K 35/30 (2013.01); C12N 2501/999 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01); C12N 2533/50 (2013.01)]

20 Claims

1. A method of making sensory neurons comprising culturing of stem cells in monolayer in chemically-defined differentiation media for sufficient duration for the cells to differentiate into sensory neurons comprising nociceptors, mechanoreceptors, and proprioceptors in a ratio similar to those found in dorsal root ganglia,

wherein, beginning on day zero or day one after plating, the stems cells are cultured in a first differentiation media comprising 5 μM to 10 μM inclusive of a TGFβ/Activin-Nodal signaling inhibitor, 0 ng/ml to 5 ng/ml of a bone morphogenic protein (BMP), and 100 nM to 500 nM inclusive of a Wnt signaling activator.