	US 12,129,475 B2
	Glycoalkaloid metabolism enyzymes (GAMES) and uses thereof
Asaph Aharoni, Tel Aviv (IL); Prashant Sonawane, Rehovot (IL); Pablo D. Cardenas, Rehovot (IL); and Burdman Saul, Rehovot (IL)
Assigned to YEDA RESEARCH AND DEVELOPMENT CO. LTD., Rehovot (IL)
Appl. No. 16/484,200
Filed by YEDA RESEARCH AND DEVELOPMENT CO. LTD., Rehovot (IL); and Yissum Research Development Company of The Hebrew University of Jerusalem Ltd., Jerusalem (IL)
PCT Filed Feb. 8, 2018, PCT No. PCT/IL2018/050142 § 371(c)(1), (2) Date Aug. 7, 2019, PCT Pub. No. WO2018/146678, PCT Pub. Date Aug. 16, 2018.
Claims priority of application No. 250538 (IL), filed on Feb. 9, 2017.
Prior Publication US 2019/0367940 A1, Dec. 5, 2019
Int. Cl. C12N 15/82 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01)

CPC C12N 15/8279 (2013.01) [C12N 9/0006 (2013.01); C12N 9/0071 (2013.01); C12N 15/8218 (2013.01); C12N 15/8243 (2013.01)]

9 Claims

1. A genetically modified plant comprising an altered content of at least one cholesterol derived compound selected from a steroidal alkaloid or a glycosylated derivative thereof, said plant comprising at least one cell having

a gene encoding a glycoalkaloid metabolism 31 (GAME31) polypeptide selected from a Solanum (S.) lycopersicum GAME31 (SlGAME31) polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 18 and a S. melongena GAME31 (SmGAME31) polypeptide comprising the amino acid sequence as set forth in SEQ ID NO: 29;

wherein said GAME 31 comprises an enhanced expression, compared to GAME31 expression in a corresponding unmodified plant;

wherein the at least one cell of said genetically modified plant has an increased content of said at least one cholesterol derived compound compared to a corresponding unmodified plant;

and

wherein said plant is a cultivated tomato plant or an aubergine plant.