US 12,129,474 B2
Composition and methods for producing tobacco plants and products having reduced tobacco-specific nitrosamines (TSNAs)
Chengalrayan Kudithipudi, Midlothian, VA (US); Dongmei Xu, Glen Allen, VA (US); and James Strickland, Richmond, VA (US)
Assigned to Altria Client Services LLC, Richmond, VA (US)
Filed by Altria Client Services LLC, Richmond, VA (US)
Filed on Mar. 13, 2023, as Appl. No. 18/182,701.
Application 18/182,701 is a continuation of application No. 16/838,762, filed on Apr. 2, 2020, granted, now 11,634,724.
Application 16/838,762 is a continuation of application No. 15/727,523, filed on Oct. 6, 2017, granted, now 10,647,989, issued on May 12, 2020.
Claims priority of provisional application 62/503,103, filed on May 8, 2017.
Claims priority of provisional application 62/405,607, filed on Oct. 7, 2016.
Prior Publication US 2023/0348925 A1, Nov. 2, 2023
Int. Cl. C12N 15/82 (2006.01); A01H 4/00 (2006.01); A24B 13/00 (2006.01); A24B 15/10 (2006.01); A24B 15/24 (2006.01); B01F 33/45 (2022.01); B01F 33/452 (2022.01); B01F 33/501 (2022.01); B01F 35/75 (2022.01); B01L 3/00 (2006.01); C07K 14/415 (2006.01); C12N 9/04 (2006.01); G01N 1/38 (2006.01); H02N 11/00 (2006.01); G01N 35/00 (2006.01); G01N 35/10 (2006.01)
CPC C12N 15/8243 (2013.01) [A01H 4/005 (2013.01); A01H 4/008 (2013.01); A24B 13/00 (2013.01); A24B 15/10 (2013.01); A24B 15/245 (2013.01); B01F 33/45 (2022.01); B01F 33/452 (2022.01); B01F 33/50112 (2022.01); B01F 35/754251 (2022.01); B01L 3/502 (2013.01); B01L 3/50825 (2013.01); C07K 14/415 (2013.01); C12N 9/0006 (2013.01); C12N 15/8205 (2013.01); C12N 15/8225 (2013.01); C12N 15/825 (2013.01); G01N 1/38 (2013.01); H02N 11/006 (2013.01); B01L 2200/0684 (2013.01); B01L 2300/043 (2013.01); B01L 2300/0672 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0403 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0478 (2013.01); B01L 2400/0683 (2013.01); G01N 2035/00534 (2013.01); G01N 35/1079 (2013.01)] 19 Claims
 
1. Cured leaf of a modified tobacco plant comprising a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs: 60 to 63, and further comprising an increased level of one or more antioxidants, wherein said reduced and increased levels are compared to a control cured leaf of an unmodified tobacco plant of the same variety when grown and cured under comparable conditions.