US 12,129,315 B2
Monomeric polysaccharide isolated from Auricularia auricula-judae, and preparation method and use thereof
Yonggang Xia, Harbin (CN); Jun Liang, Harbin (CN); Junxi Liu, Harbin (CN); Siliang Jiang, Harbin (CN); and Zihao Rao, Harbin (CN)
Assigned to Jun Liang, Harbin (CN)
Filed by Jun Liang, Harbin (CN)
Filed on Jul. 17, 2023, as Appl. No. 18/222,492.
Claims priority of application No. 202210854670.4 (CN), filed on Jul. 17, 2022.
Prior Publication US 2024/0026038 A1, Jan. 25, 2024
Int. Cl. C08B 37/00 (2006.01); A61K 31/715 (2006.01); A61P 11/00 (2006.01)
CPC C08B 37/0003 (2013.01) [A61K 31/715 (2013.01); A61P 11/00 (2018.01); C08B 37/006 (2013.01)] 1 Claim
 
1. A method for isolating a polysaccharide from an Auricularia auricula-judae (A. auricula), wherein the polysaccharide is a high-acetyl glucuronoxylogalactoglucomannan with a molecular weight of 260 kDa and an acetyl content of 18%; the polysaccharide comprises xylose (Xyl), glucuronic acid (GlcA), galactose (Gal), glucose (Glc), and mannose (Man), and the Xyl, the GlcA, the Gal, the Glc, and the Man are in a molar ratio of 3:4:1:1:11; wherein the polysaccharide comprises a chemical finely-structured repeating unit of the polysaccharide is as follows:

OG Complex Work Unit Chemistry
the method comprising:
(1) with water as an extraction solvent, conducting a heat reflux extraction (HRE) on the A. auricula to obtain an A. auricula extract-containing aqueous solution;
(2) subjecting the A. auricula extract-containing aqueous solution to an adsorption with an AB-8 macroporous adsorption resin column to remove pigments and proteins, and subjecting the AB-8 macroporous adsorption resin column to a first elution with an eluent to obtain an A. auricula extract-containing aqueous eluate; wherein the eluent is a first distilled water, and during the first elution, the first distilled water is used at an amount six times a volume of the AB-8 macroporous adsorption resin column;
(3) subjecting the A. auricula extract-containing aqueous eluate to an treatment with a 3 kDa ultrafiltration (UF) chromatographic column to obtain refined total polysaccharides of the A. auricula; and
(4) treating the refined total polysaccharides of the A. auricula with an Amber-lite FPA90-Cl− anion exchange resin column to obtain a refined polysaccharide of the A. auricula, wherein a second elution is conducted with a second distilled water in a volume four times a volume of the Amber-lite FPA90-Cl− anion exchange resin column and 1 mol/L NaCl in a volume two times the volume of the Amber-lite FPA90-Cl− anion exchange resin column, and an eluate of the distilled water is collected to obtain the refined polysaccharide of the A. auricula; and subjecting the refined polysaccharide to a refinement and a purification with a DEAE-650M anion exchange chromatographic column to obtain the polysaccharide, wherein a third elution is conducted with a third distilled water and 0.5 mol/L NaCl, and an eluate of the 0.5 mol/L NaCl is collected to obtain the polysaccharide.