US 12,128,414 B2
Automated method for performing an assay
Mark Nowakowski, San Diego, CA (US); Michael Wang, San Diego, CA (US); Robert B Cary, Santa Fe, NM (US); Hong Cai, Los Alamos, NM (US); Conrad Lindberg, Fallbrook, CA (US); Martin Bouliane, Carlsbad, CA (US); and Donald J Thomas, Vista, CA (US)
Assigned to MESA BIOTECH LLC, Carlsbad, CA (US)
Filed by Mesa Biotech, Inc., San Diego, CA (US)
Filed on Sep. 2, 2022, as Appl. No. 17/929,369.
Application 17/929,369 is a continuation of application No. 15/137,983, filed on Apr. 25, 2016, abandoned.
Claims priority of provisional application 62/322,738, filed on Apr. 14, 2016.
Claims priority of provisional application 62/152,724, filed on Apr. 24, 2015.
Prior Publication US 2024/0076724 A1, Mar. 7, 2024
Int. Cl. B01L 7/00 (2006.01); B01L 3/00 (2006.01); C12Q 1/04 (2006.01); C12Q 1/6816 (2018.01); C12Q 1/6844 (2018.01)
CPC B01L 7/52 (2013.01) [B01L 3/502723 (2013.01); B01L 3/50273 (2013.01); B01L 3/502738 (2013.01); B01L 3/502746 (2013.01); B01L 3/502761 (2013.01); B01L 3/502769 (2013.01); C12Q 1/04 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6844 (2013.01); B01L 3/502715 (2013.01); B01L 2200/0621 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/147 (2013.01); B01L 2200/16 (2013.01); B01L 2300/048 (2013.01); B01L 2300/0822 (2013.01); B01L 2300/0883 (2013.01); B01L 2300/123 (2013.01); B01L 2300/1827 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0445 (2013.01); B01L 2400/0457 (2013.01); B01L 2400/0638 (2013.01); B01L 2400/0677 (2013.01); B01L 2400/0694 (2013.01); C12Q 2531/113 (2013.01); C12Q 2560/00 (2013.01)] 19 Claims
OG exemplary drawing
 
1. An automated method of performing an assay in a microfluidic device, comprising:
introducing a sample into the microfluidic device by flowing the sample from a sample cup to a first chamber;
opening a first vent to displace air in a second chamber to an expansion chamber, thereby mixing the sample with a reagent pellet comprising amplification reagents by flowing the sample through the reagent pellet in a reagent recess to the second chamber; and
performing an amplification reaction in the second chamber directed towards a target sequence of the sample to provide an amplification product.