US 12,128,407 B2
Microfluidic chips and cartridges and systems utilizing microfluidic chips and cartridges
Elodie Sollier, Gagny (FR); Michael Lee Kochersperger, Princeton, NJ (US); Robert F. Englert, Pleasanton, CA (US); James Che, San Ramon, CA (US); Kuo-Wei Huang, Fremont, CA (US); Michael Boyce-Jacino, Titusville, NJ (US); Anatasia Neddersen, San Jose, CA (US); Anna Passernig, Campbell, CA (US); Bruce Richardson, Los Gatos, CA (US); and Insang Choi, Anyang-si (KR)
Assigned to Vortex Biosciences, Inc., Sunnyvale, CA (US)
Appl. No. 16/093,468
Filed by Vortex Biosciences, Inc., Menlo Park, CA (US)
PCT Filed Apr. 17, 2017, PCT No. PCT/US2017/027959
§ 371(c)(1), (2) Date Oct. 12, 2018,
PCT Pub. No. WO2017/181186, PCT Pub. Date Oct. 19, 2017.
Claims priority of provisional application 62/323,377, filed on Apr. 15, 2016.
Claims priority of provisional application 62/323,387, filed on Apr. 15, 2016.
Claims priority of provisional application 62/323,397, filed on Apr. 15, 2016.
Prior Publication US 2019/0168221 A1, Jun. 6, 2019
Int. Cl. B01L 3/00 (2006.01); G01N 1/10 (2006.01); G01N 15/10 (2024.01); G01N 15/14 (2024.01); G01N 35/10 (2006.01)
CPC B01L 3/502761 (2013.01) [B01L 3/502738 (2013.01); G01N 1/10 (2013.01); G01N 15/1484 (2013.01); B01L 2200/027 (2013.01); B01L 2200/0668 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/10 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/087 (2013.01); B01L 2400/0403 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0633 (2013.01); B01L 2400/084 (2013.01); G01N 2015/1006 (2013.01); G01N 35/1097 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A microfluidic chip for isolating cells or particles from a fluid, the microfluidic chip comprising:
a rigid substrate made of a material selected from poly(methyl methacrylate) (PMMA), cyclic olefin polymer (COP), cyclic olefin copolymer (COC), and ultra-violet curable resins;
an inlet that extends through the substrate;
a single outlet that extends through the substrate; and
at least one microfluidic channel disposed in the substrate between the inlet and the single outlet and having a length, the at least one microfluidic channel being in fluid communication with the inlet and the single outlet, the at least one microfluidic channel comprising a plurality of expansion regions disposed along the length of the at least one microfluidic channel in which a vortex forms in response to fluid through the at least one microfluidic channel, the plurality of expansion regions being disposed between the inlet and the single outlet, the plurality of expansion regions comprising a first expansion region, the at least one microfluidic channel being free of a runway between the inlet and the first expansion region.