US 12,128,404 B2
Microfluidic system, device and method
Wojciech Bula, Tokyo (JP); Peter Christian Sommer, Tokyo (JP); Daniel Maggs, Tokyo (JP); and Gen Suzuki, Kanagawa (JP)
Assigned to Kohler Ventures, Inc., Kohler, WI (US)
Filed by BISU, INC., Wilmington, DE (US)
Filed on Aug. 8, 2023, as Appl. No. 18/231,736.
Application 18/231,736 is a continuation of application No. 17/561,754, filed on Dec. 24, 2021, granted, now 11,717,823.
Application 17/561,754 is a continuation of application No. PCT/US2021/041706, filed on Jul. 14, 2021.
Application PCT/US2021/041706 is a continuation in part of application No. PCT/US2021/012384, filed on Jan. 6, 2021.
Claims priority of provisional application 62/989,895, filed on Mar. 16, 2020.
Claims priority of provisional application 62/957,536, filed on Jan. 6, 2020.
Prior Publication US 2023/0381777 A1, Nov. 30, 2023
Int. Cl. B01L 3/00 (2006.01); B01L 9/00 (2006.01); G01N 21/05 (2006.01)
CPC B01L 3/502715 (2013.01) [B01L 9/527 (2013.01); G01N 21/05 (2013.01); B01L 2200/0689 (2013.01); B01L 2300/0627 (2013.01); B01L 2300/069 (2013.01); B01L 2300/0887 (2013.01); B01L 2300/165 (2013.01)] 26 Claims
OG exemplary drawing
 
1. A microfluidic system comprising:
a body structure comprising
(a) a first outer layer forming a first side of the body structure;
(b) a second outer layer forming a second side of the body structure;
(c) a microfluidic assembly comprising at least a first substrate layer and disposed between the first outer layer and the second outer layer;
(d) a first port disposed through the first outer layer and in fluid communication with the microfluidic assembly; and
(e) a second port disposed through either the first outer layer or the second outer layer and adapted to be attached to a vacuum source such that a fluid flow path is defined from the first port to the second port through the microfluidic assembly;
wherein the microfluidic assembly comprises:
(i) a reaction chamber manifold and a waste reservoir; and
(ii) two or more parallel fluid flow paths between the reaction chamber manifold and the waste reservoir, wherein each fluid flow path comprises at least one reaction chamber comprising a dried film, a paper, or a gel comprising one or more test reagents, and wherein the at least one reaction chamber is adapted for an optical measurement;
wherein the reaction chamber manifold is on a sample supply inlet of each of the two or more fluid flow paths;
wherein the reaction chamber manifold on the sample supply inlet of each of the two or more fluid flow paths has a volume greater than the volume of the at least one reaction chamber; and
wherein the waste reservoir has a volume greater than the volume of the at least one reaction chamber.